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howto:determination_of_the_focal_width_with_the_focal

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howto:determination_of_the_focal_width_with_the_focal [2014/06/18 13:00]
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howto:determination_of_the_focal_width_with_the_focal [2015/01/22 15:48] (current)
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 **Note:** The data in the file is a bead that was excited via pulsed interleaved excitation (PIE) using two perpendicularly polarized lasers, which were guided through a DIC prism. This causes a displacement of the beams, which is used in dual focus FCS (2fFCS) measurements.\\ **Note:** The data in the file is a bead that was excited via pulsed interleaved excitation (PIE) using two perpendicularly polarized lasers, which were guided through a DIC prism. This causes a displacement of the beams, which is used in dual focus FCS (2fFCS) measurements.\\
-If data are acquired with a scanner controlled by the SymPhoTime 64 software (i.e. the piezo scanner in the MicroTime 200), the image dimensions are automatically correct. If the image is acquired using an external scanner, the image size has to be defined or transferred before the measurement to make sure that the measured image dimensions are correct. Otherwise, one pixel is considered to a size of 1 µm.+If data are acquired with a scanner controlled by the SymPhoTime 64 software (i.e. the piezo scanner in the [[products:​MicroTime#​MicroTime 200|MicroTime200]]), the image dimensions are automatically correct. If the image is acquired using an external scanner, the image size has to be defined or transferred before the measurement to make sure that the measured image dimensions are correct. Otherwise, one pixel is considered to a size of 1 µm.
  
 {{ determination_of_the_focal_width_with_the_focal_Image_6.png }} {{ determination_of_the_focal_width_with_the_focal_Image_6.png }}
howto/determination_of_the_focal_width_with_the_focal.txt · Last modified: 2015/01/22 15:48 by admin