howto:calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script
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howto:calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script [2014/06/17 15:36] – admin | howto:calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script [2015/11/03 14:12] (current) – admin | ||
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{{tag> tutorial howto FRET PIE analysis time-trace SymPhoTime}} | {{tag> tutorial howto FRET PIE analysis time-trace SymPhoTime}} | ||
- | ~~TOC~~ | + | ~~NOTOC~~ |
- | ====== Calculate Ratiometric Single Pair FRET Distributions Using the PIE-FRET | + | ====== Calculate Ratiometric Single Pair FRET Distributions Using PIE-FRET ====== |
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This tutorial shows step-by-step, | This tutorial shows step-by-step, | ||
+ | |||
+ | {{ youtube> | ||
**Note:** The script requires a time trace containing the fluorescence of two spectrally separated channels, one channel mainly detecting the fluorescence of the donor dye and the other channel detecting mainly the fluorescence of the acceptor dye.\\ | **Note:** The script requires a time trace containing the fluorescence of two spectrally separated channels, one channel mainly detecting the fluorescence of the donor dye and the other channel detecting mainly the fluorescence of the acceptor dye.\\ | ||
The time traces can be acquired from single molecule events in a diluted solution, where the passages of single molecules are registered as " | The time traces can be acquired from single molecule events in a diluted solution, where the passages of single molecules are registered as " | ||
- | To record such traces, usually single molecule sensitive detectors as [[glossary: | + | To record such traces, usually single molecule sensitive detectors as [[glossary: |
For the pulsed interleaved excitation ([[glossary: | For the pulsed interleaved excitation ([[glossary: | ||
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**Note:** Further information about the sample used in this measurement can be found under: J.L. Fiore et. al., Enthalpy-driven RNA-folding: | **Note:** Further information about the sample used in this measurement can be found under: J.L. Fiore et. al., Enthalpy-driven RNA-folding: | ||
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howto/calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script.txt · Last modified: 2015/11/03 14:12 by admin