adapted.\\
**Upper right:** Fluorescence Lifetime Histogram. Here the frequency of photon counts correspondin... hen applied to the lifetime graph(s) in the TCSPC histogram.\\
**Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a
at** supports PicoQuant bin files ([[glossary:pre-histogrammed_image|pre-histogrammed Image Data File]]) [[https://www.openmicroscopy.org/site/support/bio-formats5/form
====== Structure of the pre-histogrammed Image Data File======
^Data Item ^^^^Type ^Descr... 'float32'' |time resolution of the TCSPC histograms in ns |
|The following block will appe... r each ''t = 1 to <TCSPCChannels>''||||
| | | | ''HistogramData [x,y,t]'' |''int32''| counts of the TCSPC cha...
For an example in Python and Matlab see here: https://github.com/PicoQuant/pre-histogrammed-Image-Data-File
er/right:** TCPSC fitting graph showing the TCSPC histogram of the measured sample.
* In the decay form o... The TCSPC fitting window is opened and the TCSPC histograms of both files are loaded. The TCSPC histogram from the file "ATTO655_diff_FLCS-pattern.ptu is marked in gre... measurement of the dye Cy5 in water. In the TCSPC histogram it can be clearly seen that the lifetime of this
ale can be adapted.\\
**3: Fluorescence Lifetime Histogram**\\
Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\
**4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\
**5: [[glossary:TCSPC]] Decay window**\\
By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
ted.\\
- **Upper right:** Fluorescence Lifetime Histogram. Here the frequency of photon counts correspondin... the lifetime graph(s) on the lower center (TCSPC histogram).\\
- **Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red a
ale can be adapted.\\
**3: Fluorescence Lifetime Histogram**\\
Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\
**4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\
**5: [[glossary:TCSPC]] Decay window**\\
By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
ale can be adapted.\\
**3: Fluorescence Lifetime Histogram**\\
Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\
**4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\
**5: [[glossary:TCSPC]] Decay window**\\
By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
ale can be adapted.\\
**3: Fluorescence Lifetime Histogram**\\
Here the frequency of photon counts correspon... using the controls on the right of the plot. The histogram is photon weighted.\\
**4: Decay Fitting Paramete... graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\
**5: [[glossary:TCSPC]] Decay window**\\
By default, this set shows the [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0
''.etc'' container that contains at least one IRF histogram and at least one decay curve. Open the Fitting Wi... \
Clicking on (selecting) ''crv[0]'', the first histogram, the IRF in this particular case, updates the plo
ve TimeHarp260 software]]. This file contains two histograms: an [[howto:how_to_measure_the_instrument_respon... software, looks like this:
{{ :howto:et2_originalhistograms.png | Original data file content }}
Trace #0 sh... neral, such a ''.phu'' file may contain many more histograms.
It is also possible that an IRF measurement an... container:
{{ :howto:et2_workspace2.png |}}
Two histograms have been imported. They are shown as "Decay" be
ted.\\
- **Upper right:** Fluorescence Lifetime Histogram. Here the frequency of photon counts correspondin... o the lifetime graph(s) in the [[glossary:TCSPC]] histogram.\\
- **Lower center/left:** [[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red a