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- burst_duration_and_fcs_diffusion_time
- n, and photobleaching in confocal single-molecule fluorescence** ===== 1. Introduction ===== In confocal single-molecule fluorescence experiments, molecular transport through the dete... nt observables: the diffusion time extracted from fluorescence correlation spectroscopy (FCS) and the burst dura... ==== 4.1 Definition ==== In FCS, the normalized fluorescence intensity autocorrelation function is defined as