In detail, this tutorial covers:
* Different file-types used or generated by SymPhoTime64
* Image... tional options and tools otherwise hidden
===== File-Type Overview =====
^Workspace: may contain these Filetypes ^ Content of the file ^ How to get such a file in the workspace ^ How to open/process^
|''.ptu''|R
mple markup language, which tries to make the datafiles to be as readable as possible. This page contain... is behavior can be enabled in the [[doku>config]] file. Hint: If DokuWiki is a link, then it's enabled.
... ?php
/**
* Customization of the english language file
* Copy only the strings that needs to be modifie... ning the syntax for links and [[#images_and_other_files|images]] (see below) like this:
[[http://php.
}}
===== Step-by-Step Tutorial =====
Select a file and start the script
* Start [[products:SymPho... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... e going through this tutorial.
**Response:** The files of the sample workspace are displayed in the wor... _fitting_script_Image_1.png }}
* Highlight the file ''ATTO655_diff_FLCS-pattern.ptu'' by a single mou
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... e going through this tutorial.
**Response:** The files of the sample workspace are displayed in the wor... pie-fret_script_Image_1.png }}
* Highlight the file ''Cy3+Cy5_diff_PIE-FRET.ptu'' by a single mouse c
lar, please check if they use current versions of file formats and/or programming libraries before you i... s, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-in mod... ad and correlate time-tagged time resolved (TTTR) files.
* [[http://flim-analyzer.ip-korea.org/|FLIM... S Bulk Correlation Software accepting pt3 and ptu files. for fitting see https://dwaithe.github.io/FCSfi
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... e going through this tutorial.
**Response:** The files of the sample workspace are displayed in the wor... t_distributions_Image_1.png }}
* Highlight the file ''Cy3+Cy5_diff_PIE-FRET.ptu'' by a single mouse c
==== Data file import ====
Create a new workspace or open an existing one:
{{ :howto:et2_createopen.png | File menu }}
Then select Import Files. A new dialog opens, where the data file is selected:
{{ :howto:et2_fileimport.png | Selects the file
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:**\\
... e going through this tutorial.
**Response:** The files of the sample workspace are displayed in the wor... _2_species_with_Image_1.png }}
* Highlight the file ''ATTO655+Cy5_diff_FCS+FLCS.ptu'' by a single mou
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... e going through this tutorial.
**Response:** The files of the sample workspace are displayed in the wor... mage_script_Image_1.png?217 }}
* Highlight the file ''Cy5_immo_FLIM+Pol-imaging''.
{{ using_the_aniso
===== Step-by-Step Tutorial =====
==== Select a file and start the analysis ====
* Start SymPhoTime... on 2.5).
* Open the "Tutorials" workspace via **File -> Open Workspace** from the main menu.\\
**Response:​**\\ ​
The files of the "Tutorials" workspace are displayed in th... e left side of the main window.
* Highlight the file ''Hyperosmotic_Shock_MDCK_Cells.ptu'' ​by a singl
e [[http://www.picoquant.com/images/uploads/page/
files/7267/appnote_flim_fret.pdf]].
The example image ... 4 software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:**\\
... going through this tutorial.
**Response:**\\
The files of the sample workspace are displayed in the wor... onential_donors_Image_1.png }}
* Highlight the file ''CENP-labelled _cells_for_FRET.ptu'' by a single
arge }}
===== Step-by-Step =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... e going through this tutorial.
**Response:** The files of the sample workspace are displayed in the wor... the_flim_script_Image_1.png }}
* Highlight the file ''DaisyPollen_cells_FLIM.ptu'' by a single mouse
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... e going through this tutorial.
**Response:** The files of the sample workspace are displayed in the wor... moval_via_flccs_Image_1.png }}
* Highlight the file ''IBA488+IBA547_unlinked_mix.ptu'' by a single mo
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... e going through this tutorial.
**Response:** The files of the sample workspace are displayed in the wor... calculate_fccs_Image_1.png }}
* Highlight the file ''IBA488+547_crosslinked.ptu'' by a single mouse
[[https://www.picoquant.com/images/uploads/page/files/7353/appnote_diffusioncoefficients.pdf]]). This ...
===== Step-by-Step Tutorial =====
==== Select a file and generate a reference FCS curve ====
* Star... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... e going through this tutorial.
**Response:** The files of the sample workspace are displayed in the wor