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lar, please check if they use current versions of file formats and/or programming libraries before you i... s, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-in mod... ad and correlate time-tagged time resolved (TTTR) files. * [[http://flim-analyzer.ip-korea.org/|FLIM... S Bulk Correlation Software accepting pt3 and ptu files. for fitting see https://dwaithe.github.io/FCSfi
fluorescence_correlation_spectroscopy-_a_short_introduction @general
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* [[http://www.picoquant.com/images/uploads/page/files/7353/appnote_diffusioncoefficients.pdf|Diffusion... * [[http://www.picoquant.com/images/uploads/page/files/7351/appnote_quantfcs.pdf|Quantitative FCS measu... of [[http://www.picoquant.com/images/uploads/page/files/7353/appnote_diffusioncoefficients.pdf| standard
syntax @wiki
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is behavior can be enabled in the [[doku>config]] file. Hint: If DokuWiki is a link, then it's enabled. ... ?php /** * Customization of the english language file * Copy only the strings that needs to be modifie... ning the syntax for links and [[#images_and_other_files|images]] (see below) like this: [[http://php.... ted in link names. The whole [[#images_and_other_files|image]] and [[#links|link]] syntax is supported
howto @howto
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p150]] is controlled via EasyTau 2. Such ''.etc'' files can be opened directly, using the {{:howto:ff.pn... of various timing electronics may use other data file formats. Possible file name extensions are ''.thd, .nhd, .phd'' and ''.hhd'' In order to analyze such files, they have to be imported into an already existi
flim_fret_calculation_for_multi_exponential_donors @howto
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e [[http://www.picoquant.com/images/uploads/page/ files/7267/appnote_flim_fret.pdf]]. The example image ... 4 software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:**\\ ... going through this tutorial. **Response:**\\ The files of the sample workspace are displayed in the wor... onential_donors_Image_1.png }} * Highlight the file ''CENP-labelled _cells_for_FRET.ptu'' by a single
pre-histogrammed_image @glossary
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==== Structure of the pre-histogrammed Image Data File====== ^Data Item ^^^^Type ^De... | |The following block will appear in the file for each ''y = 1 to <PixY>'' |||... an example in Python and Matlab see here: https://github.com/PicoQuant/pre-histogrammed-Image-Data-File
lifetime_fitting_using_the_tcpsc_fitting_script @howto
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}} ===== Step-by-Step Tutorial ===== Select a file and start the script * Start [[products:SymPho... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... e going through this tutorial. **Response:** The files of the sample workspace are displayed in the wor... _fitting_script_Image_1.png }} * Highlight the file ''ATTO655_diff_FLCS-pattern.ptu'' by a single mou
lifetime-fitting_using_the_flim_script @howto
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===== Step-by-step Tutorial ===== ==== Select a file and start the analysis ==== * Start SymPhoTime ... rsions). * Open the "Tutorials" workspace via **File -> Open Workspace** from the main menu.\\ **Response:​**\\ ​ The files of the sample workspace are displayed in the wor... e left side of the main window. * Highlight the file ''​Kidney_Cells_FLIM.ptu'' ​by a single mouse cli
roi_fitting_using_the_flim_script @howto
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arge }} ===== Step-by-Step ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... e going through this tutorial. **Response:** The files of the sample workspace are displayed in the wor... the_flim_script_Image_1.png }} * Highlight the file ''DaisyPollen_cells_FLIM.ptu'' by a single mouse
update @howto
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===== Step-by-step Tutorial ===== ==== Select a file and start the analysis ==== * Start SymPhoTime... rsions). * Open the “Tutorials” workspace via **File -> Open Workspace** from the main menu.\\ **Response:​**\\ ​ The files of the sample workspace are displayed in the wor... e left side of the main window. * Highlight the file ''​Kidney_Cells_FLIM.ptu'' ​by a single mouse cli
how_to_work_with_the_instrument_response_function_irf @howto
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ng used in the analysis. Therefore, highlight the file with the measured IRF (single mouse click on the .ptu-file)\\ An example measured IRF can be found in Tutori... |}} 6. Click on “Export Decay as IRF”. Rename the file accordingly. The IRF can now be used for the fitt... laser settings (Please see `System Specification File` which is left by installation in your lab. The m
visualizing_dynamics_using_the_multiframe-flim_script @howto
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===== Step-by-Step Tutorial ===== ==== Select a file and start the analysis ==== * Start SymPhoTime... on 2.5). * Open the "Tutorials" workspace via **File -> Open Workspace** from the main menu.\\ **Response:​**\\ ​ The files of the "Tutorials" workspace are displayed in th... e left side of the main window. * Highlight the file ''Hyperosmotic_Shock_MDCK_Cells.ptu'' ​by a singl
lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
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===== Step-by-Step Tutorial ===== ==== Select a file and start the analysis ==== * Start SymPhoTime... on 2.5). * Open the "Tutorials" workspace via **File -> Open Workspace** from the main menu. **Response:**\\ The files of this workspace are displayed in the workspace... left side of the main window. * Highlight the file ''MicroBeads.ptu'' by a single mouse click.
configuring_symphotime64_after_installation @support
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Settings-> Save User Configuration. * Save the file to a folder so that you can have it as a back-up ... . You can create a new workspace or load goint to File→… {{ :support:workspace.jpg?200 |}} ====Bright/D
data_file_import @howto
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==== Data file import ==== Create a new workspace or open an existing one: {{ :howto:et2_createopen.png | File menu }} Then select Import Files. A new dialog opens, where the data file is selected: {{ :howto:et2_fileimport.png | Selects the file
create_time_gated_image
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symphotime_tips_and_tricks @howto
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calculate_fccs_trace_with_the_grouped_fcs_script @howto
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phasor_analysis @howto
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measurement_hardware_instrumentation
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using_the_anisotropy_image_script @howto
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measuring_quantum_yield @howto
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pycorrfit @software
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calculate_and_fit_fcs_traces_with_the_fcs_script @howto
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calculate_ratiometric_fret-images @howto
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lifetime_fitting_using_the_flim_analysis @howto
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registering_new_scripts @howto
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bdf @glossary
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tttr @glossary
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t3-mode @glossary
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t2-mode @glossary
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determination_of_the_focal_width_with_the_focal @howto
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intensity_time_trace_analysis @howto
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using_the_antibunching_script @howto
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pattern_matching @howto
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separation_of_2_species_with @howto
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flimfit @software
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