xample image used is an image showing four cells, two of them only transfected with the CENP-B-Cerulean complex, the other two transfected with both constructs. Therefore, the ... ted.
**Note:**\\
Now four cells are visible, the two nuclei on the left have longer Fast lifetimes then the other two. These are the cells transfected only with the do
as for fluorescence measurement
</code>
==== Two photon excitation (TPE) ====
Do not attempt to r... ysis of time domain measurements =====
There are two major ways of compensating IRF effects:
*cor
Image_5.png?600 }}
**Note:** The window contains two different regions:\\
- **Left:** Fitting and an... splayed in the script. For illustration, mark the two files ''Cy5_diff_IRF+FLCS-pattern.ptu'' and ''ATT... . In our example, it is of course meaningless, as two different dyes were fitted.
* Place the mouse
tubul_magicwand20_3.png |}}
* Repeat the last two steps in order to select the cell nuclei as the l... del should be used for ROI 0.\\
* Try the last two steps with "ROI 1".\\
{{ howto:tubul_3expo_3.png...
{{ howto:showall.png |}}
* Uncheck the first two datasets on top of the parameter table and press
s of the twist angle and polarization between the two twisted dithienothiophenes of the mechanophore.\\... et 2), press "Show All" button, uncheck the first two datasets on top of the parameter table and press
each of these parameters individually.
There are two ways to define the new threshold. You can use the... ount rates there
is an increasing likelihood that two or more such detector pulses will overlap and mer... constant of the systematic errors.
* Try last two steps with the ROI 1.
{{ howto:monoexpo_roi1.pn... on is not sufficient. Therefore, we carry on with two components.
{{ howto:biexpo_roi1.png |}}
**
native TimeHarp260 software]]. This file contains two histograms: an [[howto:how_to_measure_the_instrum... measurement and a decay measurement are stored in two separate ''.phu'' files. This is no problem, but ... data container:
{{ :howto:et2_workspace2.png |}}
Two histograms have been imported. They are shown as ... \
\\
Unlike ''Decay_Coumarin_6.phu'' containing two histograms, both ''Anthracene_EtOH.phu'' and ''IR
each of these parameters individually.
There are two ways to define the new threshold. You can use the... n be found in the Binding histogram below showing two distinct peaks.
* On the lower right, the Bindi
ethod to determine molecular interactions between two molecules labeled with different dyes. Usually, the dyes attached to the two molecules of interest differ in their absorption ... , a negative calibration probe, i.e. a mixture of two single labeled compounds needs to be measured. To