for_multi-exponential_donors_Image_2.png? }}
* Select the "Analysis" tab and in there, open the drop do... and open the context menu by a right mouse click. Select the "Free ROI" tool.
{{ flim-fret-calculation_for... 0 }}
* In the decay form on the lower left, select n-exponential reconvolution as fitting model.
{{ ... of the different parameters.
* Under "Decay" select "ROI 0".
**Response:**\\
The TCSPC curve of ROI 0
VV8?large }}
===== Step-by-Step Tutorial =====
Select a file and start the script
* Start [[products... sing_the_tcpsc_fitting_script_Image_2.png }}
* Select the "Analysis" tab and in there, open the drop do... ured sample.
* In the decay form on the left, select n-exponential reconvolution as fitting model.
**R... a right mouse click to open the context menu and select "Copy" or simple use "<CRTL>" + "C" to copy the f
formed.
===== Step-by-step Tutorial =====
==== Select a file and start the analysis ====
* Start SymP... ey_Cells_FLIM.ptu'' by a single mouse click.
* Select the **"Analysis"** tab and in there, open the *... st by adapting "Min" and "Max" values.
==== Select ROIs ====
* Open the "Region of Interest" drop... n you are content with the selection, press OK to select these pixels of the image as Region of Interest (
vLQW4nY?large }}
===== Step-by-Step =====
==== Select a file and start the script ====
* Start [[pro... fitting_using_the_flim_script_Image_2.png }}
* Select the "Analysis"-tab and in there, open the drop do... ime contrasts in the pollen become visible.
==== Select 3 Regions of Interest (ROI) ====
* Keep the m... ing_using_the_flim_script_Image_7.png?349 }}
* Select a ROI tool in the context menu, e.g. the "FreeROI
rmed.\\
===== Step-by-step Tutorial =====
==== Select a file and start the analysis ====
* Start Sym... ey_Cells_FLIM.ptu'' by a single mouse click.
* Select the **"Analysis"** tab and in there, open the *... st by adapting "Min" and "Max" values.
==== Select a file and start the analysis ====
* In the FL... nu.\\
- If you want to start again from scratch select "Select all as ROI".\\
* In this step, as an e
peak is above 100000, so fine for this step)\\
3. Select the proper detection channel (1 or 2) and Click”Calculate Decay”. The decay is then updated.\\
4. Select the decay range by Setting a `Time Gate` (move th... Decay`.
As a result you will see:
{{ howto:irf_select_channel_timegate.png?800 |}}
6. Click on “Export ... IRF also depends on the detection wavelength).
Select the linear range for measured curve (on top right
f86c23]].
===== Step-by-Step Tutorial =====
==== Select a file and start the analysis ====
* Start Sym... ck_MDCK_Cells.ptu'' by a single mouse click.
* Select the **"Analysis"** tab and in there, open the *... ram.\\
==== Define image stack options and select ROI ====
* In parameter panel, enter a frame ... k on the FastFLIM image and from the context menu select "FreeROI" tool.\\
* In order to quantify tensi
6c23]].
===== Step-by-Step Tutorial =====
==== Select a file and start the analysis ====
* Start Sym... 'MicroBeads.ptu'' by a single mouse click.
* Select the **"Analysis"** tab and in there, open the **"... t by adapting "Min" and "Max" values.
==== Select ROIs ====
* On top left, under Region of Int... d Fast Lifetime color code. This will help you to select your ROI or threshold easier.
{{ howto:adaptcont
===== Reconvolution fit =====
Select an ''.etc'' container that contains at least one IRF histogram and a... order to perform a reconvolution fit, we need to select and then assign a curve as an IRF and then change
{ :howto:et2_createopen.png | File menu }}
Then select Import Files. A new dialog opens, where the data ... multiple data files is easy. Use CTRL + Click to select multiple files:
{{ :howto:et2_fileimport2.png |}
a time can be entered in the field above. Here we select the photons, which arrive roughly with the laser ... in the main control window (in fact, you can also select the time gate there, but as the window is smaller... time-gated intensity image, set to greyscale and select "Events" as parameter to show.
{{:flim-image_aft... time-gated image should be selected, either just select a new time gate as described above and repeat the
large }}
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[pro... lick.
{{ visualizing_dynamics_Image_2.png }}
* Select the "Analysis" tab and in there, open the drop do... cess indicated by blurring of certain GUVs.
==== Select the Processing Options and create and export an i... be used in all images of the series, first check "Select in all Frames" and then mark the ROI.
{{ visuali