for_multi-exponential_donors_Image_2.png? }}
* Select the "Analysis" tab and in there, open the drop do... and open the context menu by a right mouse click. Select the "Free ROI" tool.
{{ flim-fret-calculation_for... **\\
For more information about the different ROI selection tools, check the tutorial [[howto:roi_fitting_... he left mouse button activated while circling the selected part of the image. Then release the left mouse
This tutorial shows step-by-step, how to fit a selected model to a decay curve in order to get the life... VV8?large }}
===== Step-by-Step Tutorial =====
Select a file and start the script
* Start [[products... sing_the_tcpsc_fitting_script_Image_2.png }}
* Select the "Analysis" tab and in there, open the drop do... ured sample.
* In the decay form on the left, select n-exponential reconvolution as fitting model.
**R
formed.
===== Step-by-step Tutorial =====
==== Select a file and start the analysis ====
* Start SymP... ey_Cells_FLIM.ptu'' by a single mouse click.
* Select the **"Analysis"** tab and in there, open the *... meters**\\
Here the lifetime fitting model can be selected. Fitting is then applied to the lifetime graph(... st by adapting "Min" and "Max" values.
==== Select ROIs ====
* Open the "Region of Interest" drop
vLQW4nY?large }}
===== Step-by-Step =====
==== Select a file and start the script ====
* Start [[pro... fitting_using_the_flim_script_Image_2.png }}
* Select the "Analysis"-tab and in there, open the drop do... ime contrasts in the pollen become visible.
==== Select 3 Regions of Interest (ROI) ====
* Keep the m... ing_using_the_flim_script_Image_7.png?349 }}
* Select a ROI tool in the context menu, e.g. the "FreeROI
rmed.\\
===== Step-by-step Tutorial =====
==== Select a file and start the analysis ====
* Start Sym... ey_Cells_FLIM.ptu'' by a single mouse click.
* Select the **"Analysis"** tab and in there, open the *... meters**\\
Here the lifetime fitting model can be selected. Fitting is then applied to the lifetime graph(... st by adapting "Min" and "Max" values.
==== Select a file and start the analysis ====
* In the FL
wto:irf_decay_before_timegate.png?800 |}}
(Before selecting a detection channel the peak is above 100000, so fine for this step)\\
3. Select the proper detection channel (1 or 2) and Click”Calculate Decay”. The decay is then updated.\\
4. Select the decay range by Setting a `Time Gate` (move th... better, double click on the graph with the gating selection. It is opened in a new window with you can enl
f86c23]].
===== Step-by-Step Tutorial =====
==== Select a file and start the analysis ====
* Start Sym... ck_MDCK_Cells.ptu'' by a single mouse click.
* Select the **"Analysis"** tab and in there, open the *... meters**\\
Here the lifetime fitting model can be selected. Fitting is then applied to the lifetime graph(... ram.\\
==== Define image stack options and select ROI ====
* In parameter panel, enter a frame
6c23]].
===== Step-by-Step Tutorial =====
==== Select a file and start the analysis ====
* Start Sym... 'MicroBeads.ptu'' by a single mouse click.
* Select the **"Analysis"** tab and in there, open the **"... meters**\\
Here the lifetime fitting model can be selected. Fitting is then applied to the lifetime graph(... t by adapting "Min" and "Max" values.
==== Select ROIs ====
* On top left, under Region of Int
rs====
* **TCSPC **: Use the drop down menu to select the proper TCSPC module then click "Init.TCSPC".
... * Def.: Default value. This value will be selected when the laser is selected via the SymPhoTIme64 as a statrting value for the intensity of the driver.
===== Reconvolution fit =====
Select an ''.etc'' container that contains at least one IRF histogram and a... order to perform a reconvolution fit, we need to select and then assign a curve as an IRF and then change... :howto:et_fitwindow1.png |}}
\\
\\
Clicking on (selecting) ''crv[0]'', the first histogram, the IRF in t
{ :howto:et2_createopen.png | File menu }}
Then select Import Files. A new dialog opens, where the data file is selected:
{{ :howto:et2_fileimport.png | Selects the file to be imported }}
The file selected above is a sample data file, ''Decay_Coumarin_6.phu'' inst
, which can be amplified
{{:flim-image_time-gate_selection.png|}}
* Now a time gat can be defined by m... a time can be entered in the field above. Here we select the photons, which arrive roughly with the laser pulse. The green region in the image is the selected region.
{{:flim-image_time-gate_selection2.png|}}
* Click "OK" to close the window. Now the selecti