{{tag>howto tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}}
====== Lifetime-Fitting Using the ... e FLIM analysis. Thereby, a new Window opens:
{{ howto:kidneyfullscreen3.png |}}
**Note:**\\
The window... I" checkbox and click "ROI from threshold".\\
{{ howto:activateroi.png |}}
**Response:**\\
A large win... a threshold for an image analysis visually.
{{ howto:selectthreshold_kidney3.png |}}
**Note:**\\
Lef
{{tag> howto FLIM imaging ROI analysis tutorial SymPhoTime}}
~~NOTOC~~
====== ROI Fitting Using the FLI... pt. Also check the script explaining pixel fitting ([[howto:Lifetime Fitting using the FLIM Analysis]]).
{{tag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade}}
====== ROI Fitting Using the FLIM Analys... rt the FLIM script by clicking on "Start".\\
{{ howto:startflim.png?200 |}}
**Response:**\\
The FL... lls_FLIM.ptu''. Thereby, a new Window opens:
{{ howto:kidneyfullscreen3.png |}}
**Note:**\\
The windo... channel 4 - and press "Calculate FastFLIM".\\
{{ howto:detectionchannel.png?400 |}}
**Response:**\\
Th
{{tag>howto IRF tutorial analysis MicroTime LSM_upgrade}}
====== How to Work with Instrument Response F... ator the timing resolution of the instrument.
{{ howto:irf.png?500 |}}
The IRF width is approx. a quad... re described here: https://www.tcspc.com/doku.php/howto:how_to_measure_the_instrument_response_function_i... ral strategies are possible which are described [[howto:how_to_measure_the_instrument_response_function_i
{{tag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade reconvolution fitting}}
====== Lifetime-Fi... art the FLIM analysis by clicking on "Start".
{{ howto:flimgui.png?200 |}}
**Response:**\\
The FLIM an... o the data file. Thereby, a new Window opens:
{{ howto:sptwindows_rapidflim.png |}}
**Note:**\\
The w... etime range for histogram on the right hand.
{{ howto:contrastadaptation2.png?600 |}}
* Set Proper
{{tag>howto MicroTime200 microscopy backreflection alignment }}
====== Image on the beam diagnostics came... just tilting, is necessary to achieve that.)
{{:howto:backreflection_defocused1.mp4|}}
Adding a drop o... how this is used also by watching the tutorial [[howto:exchange_dichroic_mt200|How to exchange the main