{{tag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade reconvolution fitting}}
====== Lifetime-Fi... art the FLIM analysis by clicking on "Start".
{{ howto:flimgui.png?200 |}}
**Response:**\\
The FLIM an... o the data file. Thereby, a new Window opens:
{{ howto:sptwindows_rapidflim.png |}}
**Note:**\\
The w... etime range for histogram on the right hand.
{{ howto:contrastadaptation2.png?600 |}}
* Set Proper
ications =====
==== Fluorescence Lifetime ====
[[howto:lifetime_fitting_using_the_tcpsc_fitting_script| ... video_tutorials| FLIM by Philippe Bastiaens]]
[[howto:how_to_measure_the_instrument_response_function_i... asure the Instrument Response function (IRF)]]
[[howto:lifetime_fitting_using_the_flim_analysis|Lifetime fitting Using the FLIM analysis software]]
[[howto:roi_fitting_using_the_flim_script|ROI fitting usi
{{tag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade}}
====== ROI Fitting Using the FLIM Analys... rt the FLIM script by clicking on "Start".\\
{{ howto:startflim.png?200 |}}
**Response:**\\
The FL... lls_FLIM.ptu''. Thereby, a new Window opens:
{{ howto:kidneyfullscreen3.png |}}
**Note:**\\
The windo... channel 4 - and press "Calculate FastFLIM".\\
{{ howto:detectionchannel.png?400 |}}
**Response:**\\
Th
{{tag>tutorial howto FRET FLIM imaging analysis SymPhoTime}}
~~NOTOC~~
====== FLIM-FRET Calculation fo... ce panel on the left side of the main window.
{{ :howto:flim-fret-1expd_samples_ws.png |}}
* Highlight... T_GFP and mRFP.ptu'' by a single mouse click.
{{ :howto:flim-fret-1expd_gret_gfp_and_mrfp.png |}}
* Se... in there, open the drop down menu "Imaging".
{{ :howto:flim-fret-1expd_analysis_imaging.png?500 |}}
**N
{{tag>howto tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}}
====== Lifetime-Fitting Using the ... e FLIM analysis. Thereby, a new Window opens:
{{ howto:kidneyfullscreen3.png |}}
**Note:**\\
The window... I" checkbox and click "ROI from threshold".\\
{{ howto:activateroi.png |}}
**Response:**\\
A large win... a threshold for an image analysis visually.
{{ howto:selectthreshold_kidney3.png |}}
**Note:**\\
Lef
g> FT300 easyTau Quantum_Yield Integrating_Sphere howto tutorial}}
====== Measuring the Quantum Yield wi... an indexed lid to ensure proper orientation.
{{ :howto:2013-12-19_at_14-30-03.jpg?600 |}}
* Use the p... he sample holder into the integrating sphere
{{ :howto:2013-12-19_at_14-35-37.jpg?600 |}}
* Select ei... ight originating from the sphere's surface).
{{ :howto:2013-12-19_at_14-35-27.jpg?600 |}}
* Switch on
me 300#Time Resolved Emission Spectra (TRES)]]
[[howto:measuring_quantum_yield|Measuring the Quantum Yie... oftware:EasyTau#Scripts | EasyTau Scripts]]
/*[[howto:how_to_measure_the_instrument_response_function_i... Kurt Thorn]]
[[Beampath of the Zeiss LSM880]]
[[howto:check_overlap_of_different_color_confocal_volumes... overlap of different color confocal volumes]]
[[howto:determination_of_the_focal_width_with_the_focal|D
ate a new workspace or open an existing one:
{{ :howto:et2_createopen.png | File menu }}
Then select I... alog opens, where the data file is selected:
{{ :howto:et2_fileimport.png | Selects the file to be impor... ftware]]. This file contains two histograms: an [[howto:how_to_measure_the_instrument_response_function_i... ned in the native software, looks like this:
{{ :howto:et2_originalhistograms.png | Original data file c
{{tag>howto IRF tutorial analysis MicroTime LSM_upgrade}}
====== How to Work with Instrument Response F... ator the timing resolution of the instrument.
{{ howto:irf.png?500 |}}
The IRF width is approx. a quad... re described here: https://www.tcspc.com/doku.php/howto:how_to_measure_the_instrument_response_function_i... ral strategies are possible which are described [[howto:how_to_measure_the_instrument_response_function_i
{{tag>Howto Analysis Tutorial SymPhoTime Tips&Tricks}}
====== SymPhoTime 64 Analysis Tips and Tricks... ice direction"** in the following GUIs \\ \\
{{:howto:slice_direction.jpg?250|}} \\ \\
* FLIM
* ... icking on the **"Resolution estimation"** tab. {{:howto:resestimationflim.jpg?250|}} \\ \\ or \\ \\ {{:howto:pattern_resolution_est.jpg?250|}}
Let us take a
====== HowTo's and Tutorials ======
===== Instrumentation =====
==== Fluorometer ====
{{topic>howto support +FT300 -sample -analysis -draft &nodate&nouser}}
==== Microscopy ====
{{topic>howto -FT300 -sample -analysis -draft &nodate&nouser}}
... .etc'' files can be opened directly, using the {{:howto:ff.png|}} button.
* It is also possible to use
cay curve. Open the Fitting Window by pressing {{:howto:ff.png?40|}}.
{{ :howto:et_sel1.png |}}
\\
\\
The last curve in the container, ''crv[1]'', a decay c... an IRF and then change the fitting modality.
{{ :howto:et_fitwindow1.png |}}
\\
\\
Clicking on (select... e type" can be changed from "Decay" to "IRF".
{{ :howto:et_fitwindow2.png |}}
\\
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The plot is updat