Enter information about the sample and the solvent in the “Sample”-Tab (in this example: Anthracene solved in Ethanol).
Additional information about the measurement or the sample may be stored in the “Comment”-Field:
The system will perform a quick measurement of the emission spectrum and change the attenuator settings for an optimal signal countrate across the chosen wavelength range.
Once the optimization has finished the “Start Optimization” - Button the count rate displayed in the bar above the optimization-button.
NOTE: On the right side Easytau 2 shows a graphical preview of the emission spectrum. If you want to change the scanning range, you can go back to the “Sample Setup” - Tab by clicking the “Sample Setup” - Button. There, change the measurement parameters as desired and restart the optimization process. You'll have to re-do the optimization if you do this, before you can proceed to the “Acuire Spectrum”-page.
If your signal is relatively low in comparison to the dark counts of the system, a “blank”, i.e. an measurement of the buffer only can be acquired in order to properly subtract the background. This is mainly important with detectors with higher dar count rates, e.g. IR-PMTs. If you don't want to acquire a blank, directly step to the “Save & Exit”-page. To measure a blank, click on the “Acquire Blank” - Button.
Turn off the laser by turning the key of the laser driver from the “Laser Active” position to the “Locked” position.
Exchange the Sample in the chamber with the blank sample (here spectral grade Ethanol).
Close the lid and turn on the laser by turning the key of the laser driver from the “Locked” position to the “Laser Active” position.
Click the “Start” - Button to start the acquisition of the blank emission spectrum.
The grey graph overlaying the spectrum shows the normalized spectrum of the blank sample.
There is also a red graph which is not normalized. In this example the photons counted are so low that this graph can not be displayed.
When the acquisition is finished click the “Save & Exit” - Button.