https://www.tcspc.com/ 2026-04-11T19:47:27+00:00 https://www.tcspc.com/ https://www.tcspc.com/lib/exe/fetch.php/logo.png text/html 2025-03-11T08:19:29+00:00 Anonymous (anonymous@undisclosed.example.com) https://www.tcspc.com/doku.php/faq:novaflim_calculated_irf?rev=1741681169&do=diff <table><tr><th colspan="2" width="50%">2025/02/18 13:31</th><th colspan="2" width="50%">current</th></tr><tr><td class="diff-blockheader" colspan="2">Line 1:</td> <td class="diff-blockheader" colspan="2">Line 1:</td> </tr> <tr><td class="diff-lineheader">&#160;</td><td class="diff-context">====== NovaFLIM Calculated IRF ======</td><td class="diff-lineheader">&#160;</td><td class="diff-context">====== NovaFLIM Calculated IRF ======</td></tr> <tr><td colspan="2">&#160;</td><td class="diff-lineheader">+</td><td class="diff-addedline">{{tag&gt; faq software novaflim IRF}}</td></tr> <tr><td colspan="2">&#160;</td><td class="diff-lineheader">+</td><td class="diff-addedline"></td></tr> <tr><td class="diff-lineheader">&#160;</td><td class="diff-context"></td><td class="diff-lineheader">&#160;</td><td class="diff-context"></td></tr> <tr><td class="diff-lineheader">&#160;</td><td class="diff-context">In the Nova Flim the IRF is parameterized as a combination of two Gaussian curves (a total of 5 fit parameters). These parameters are adjusted during Initial Fit.&#160;</td><td class="diff-lineheader">&#160;</td><td class="diff-context">In the Nova Flim the IRF is parameterized as a combination of two Gaussian curves (a total of 5 fit parameters). These parameters are adjusted during Initial Fit.&#160;</td></tr> <tr><td class="diff-blockheader" colspan="2">Line 22:</td> <td class="diff-blockheader" colspan="2">Line 24:</td> </tr> <tr><td class="diff-lineheader">&#160;</td><td class="diff-context">&#160; * Height of Gaussian curve 2 (limited to the height of Gaussian curve 1)</td><td class="diff-lineheader">&#160;</td><td class="diff-context">&#160; * Height of Gaussian curve 2 (limited to the height of Gaussian curve 1)</td></tr> <tr><td class="diff-lineheader">&#160;</td><td class="diff-context"></td><td class="diff-lineheader">&#160;</td><td class="diff-context"></td></tr> <tr><td class="diff-lineheader">-</td><td class="diff-deletedline">This model is the result of extensive internal development, testing, and refinement. By optimizing these parameters, the IRF model can accurately represent the measured response of the system.</td><td class="diff-lineheader">+</td><td class="diff-addedline"><strong class="diff-mark">The IRF is determined only during the initial TCSPC fit, which means it may vary depending on the different exponential components. During the pixelwise fitting in FLIM, this IRF is applied without any modifications. Consequently, all image points are fitted using the same fixed IRF established during the initial fit. </strong></td></tr> <tr><td colspan="2">&#160;</td><td class="diff-lineheader">+</td><td class="diff-addedline"><strong class="diff-mark"> </strong></td></tr> <tr><td colspan="2">&#160;</td><td class="diff-lineheader">+</td><td class="diff-addedline">This model is the result of extensive internal development, testing, and refinement. By optimizing these parameters, the IRF model can accurately represent the measured response of the system.&#160;</td></tr> <tr><td class="diff-lineheader">&#160;</td><td class="diff-context"></td><td class="diff-lineheader">&#160;</td><td class="diff-context"></td></tr> <tr><td class="diff-lineheader">&#160;</td><td class="diff-context"></td><td class="diff-lineheader">&#160;</td><td class="diff-context"></td></tr> </table>