Time-Resolved Fluorescence Wiki
https://www.tcspc.com/
2026-04-21T11:50:06+00:00Time-Resolved Fluorescence Wiki
https://www.tcspc.com/
https://www.tcspc.com/lib/exe/fetch.php/logo.pngtext/html2015-02-10T16:34:08+00:00Anonymous (anonymous@undisclosed.example.com)bifl
https://www.tcspc.com/doku.php/glossary:bifl?rev=1423586048&do=diff
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<tr><td class="diff-lineheader"> </td><td class="diff-context">BIFL stands for **B**urst **I**ntegrated **F**luorescence **L**ifetime: The photon events of a complete burst are accumulated into a [[TCSPC]] histogram which is used for lifetime analysis methods afterwards. Usually a burst is defined as a time interval for which the intensity of a time trace or [[MCS]] measurement is above a chosen threshold. Evaluation of all bursts present in an [[MCS]] trace, for example, results in a lifetime histogram.</td><td class="diff-lineheader"> </td><td class="diff-context">BIFL stands for **B**urst **I**ntegrated **F**luorescence **L**ifetime: The photon events of a complete burst are accumulated into a [[TCSPC]] histogram which is used for lifetime analysis methods afterwards. Usually a burst is defined as a time interval for which the intensity of a time trace or [[MCS]] measurement is above a chosen threshold. Evaluation of all bursts present in an [[MCS]] trace, for example, results in a lifetime histogram.</td></tr>
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<tr><td class="diff-lineheader">-</td><td class="diff-deletedline">BIFL is supported by the [[SymPhoTime]] software, alongside with a variety of burst related techniques.</td><td class="diff-lineheader">+</td><td class="diff-addedline">BIFL is supported by the [[<strong class="diff-mark">software:</strong>SymPhoTime]] software, alongside with a variety of burst related techniques.</td></tr>
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</table>text/html2015-06-03T15:09:55+00:00Anonymous (anonymous@undisclosed.example.com)pile-up_effect
https://www.tcspc.com/doku.php/glossary:pile-up_effect?rev=1433344195&do=diff
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<tr><td class="diff-lineheader">-</td><td class="diff-deletedline">{{tag>pile-up <strong class="diff-mark">TCSPC</strong>}} </td><td class="diff-lineheader">+</td><td class="diff-addedline">{{tag> <strong class="diff-mark">TCSPC </strong>pile-up <strong class="diff-mark">dead_time</strong>}} </td></tr>
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<tr><td class="diff-lineheader"> </td><td class="diff-context">====== Pile-Up Effect ======</td><td class="diff-lineheader"> </td><td class="diff-context">====== Pile-Up Effect ======</td></tr>
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</table>text/html2014-01-28T13:06:31+00:00Anonymous (anonymous@undisclosed.example.com)tcspc
https://www.tcspc.com/doku.php/glossary:tcspc?rev=1390914391&do=diff
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<tr><td class="diff-lineheader"> </td><td class="diff-context">====== TCSPC ======</td><td class="diff-lineheader"> </td><td class="diff-context">====== TCSPC ======</td></tr>
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<tr><td class="diff-lineheader">-</td><td class="diff-deletedline">TCSPC stands for **T**ime **C**orrelated **S**ingle **P**hoton **C**ounting: A stream of photons is recorded, each photon with respect to a (often periodical) signal, e.g. a repeated laser pulse. The single photon events are sorted into histogram channels that correspond to the time that elapsed since the last excitation pulse. It is a time domain method <strong class="diff-mark">of </strong>investigating time resolved fluorescence<strong class="diff-mark">. [[Products:TimeHarp 260]], [[Products:HydraHarp 400]] and [[Products:PicoHarp 300]] use TCSPC</strong>. </td><td class="diff-lineheader">+</td><td class="diff-addedline">TCSPC stands for **T**ime **C**orrelated **S**ingle **P**hoton **C**ounting: A stream of photons is recorded, each photon with respect to a (often periodical) signal, e.g. a repeated laser pulse. The single photon events are sorted into histogram channels that correspond to the time that elapsed since the last excitation pulse. It is a time domain method <strong class="diff-mark">for </strong>investigating time resolved fluorescence.</td></tr>
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