Time-Resolved Fluorescence Wiki

contributing

Anonymous (anonymous@undisclosed.example.com) — 2018-02-19T08:06:13+00:00

2014/04/11 07:32		current
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	Links that don't have an article yet will be shown in red. This makes it easy for others to just start this article.		Links that don't have an article yet will be shown in red. This makes it easy for others to just start this article.

		+	====== Most searched words ======
		+	~~SEARCHCLOUD~~

	====== Improve an article ======		====== Improve an article ======

community_news

Anonymous (anonymous@undisclosed.example.com) — 2020-05-04T12:11:21+00:00

2020/04/14 11:40		current
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	===== News from the community=====		===== News from the community=====
		+	* 29.04.20 [[https://www.fret.community/announcement-protein-folding-and-dynamics-webinar/\|Protein folding and dynamics Webinar-Series.]] \\ \\
	* 14.04.20 [[https://analyticalscience.wiley.com/do/10.1002/was.00050003/full/bioimagedataanalysis.pdf\|Bioimage Data Analysis.]] New Expanded version of a nice open-source introduction. \\ \\		* 14.04.20 [[https://analyticalscience.wiley.com/do/10.1002/was.00050003/full/bioimagedataanalysis.pdf\|Bioimage Data Analysis.]] New Expanded version of a nice open-source introduction. \\ \\
-	* 14.04.20 [[https://science.sciencemag.org/content/368/6486/78\|De novo design of protein logic gates.]] Fundamendal enzymology work. Chen et al. describe the design of logic gates that can regulate protein association. The gates were built from small, designed proteins that all have a similar structure but where one module can be designed to interact specifically with another module. Using monomers and covalently connected monomers as inputs and encoding specificity through designed hydrogen-bond networks allowed the construction of two-input or three-input gates based on competitive binding. \\ \\	+	* 14.04.20 [[https://science.sciencemag.org/content/368/6486/78\|De novo design of protein logic gates.]] Fundamental enzymology work. Chen et al. describe the design of logic gates that can regulate protein association. The gates were built from small, designed proteins that all have a similar structure but where one module can be designed to interact specifically with another module. Using monomers and covalently connected monomers as inputs and encoding specificity through designed hydrogen-bond networks allowed the construction of two-input or three-input gates based on competitive binding. \\ \\
	* 31.03.20 FRET community has a new advisory board, [[https://www.fret.community/\|FRET community]]. Happy that Dr. Marcelle König , a senior scientist in PicoQuant was elected. \\ \\		* 31.03.20 FRET community has a new advisory board, [[https://www.fret.community/\|FRET community]]. Happy that Dr. Marcelle König , a senior scientist in PicoQuant was elected. \\ \\
	* 31.03.20 Extremely helpful reference paper about confocal microscopy. [[https://www.nature.com/articles/s41596-020-0313-9\|Tutorial: guidance for quantitative confocal microscopy]]. Available as a poster [[https://www.nature.com/articles/s41596-020-0307-7\|here.]] \\ \\		* 31.03.20 Extremely helpful reference paper about confocal microscopy. [[https://www.nature.com/articles/s41596-020-0313-9\|Tutorial: guidance for quantitative confocal microscopy]]. Available as a poster [[https://www.nature.com/articles/s41596-020-0307-7\|here.]] \\ \\

supported_th260_pc

Anonymous (anonymous@undisclosed.example.com) — 2017-09-20T15:42:57+00:00

2017/04/07 18:03		current
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		+	/*
	{{tag> PC TH260 Harps TCSPC}}		{{tag> PC TH260 Harps TCSPC}}
	~~TOC~~		~~TOC~~
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	\|HP ENVY 700-056 \|AMD A75 FCH\| \|		\|HP ENVY 700-056 \|AMD A75 FCH\| \|
	\|Dell Precision T3400 \|Intel X38 \| Installed in SLOT 3 , PCIe 8x(4,1)\|		\|Dell Precision T3400 \|Intel X38 \| Installed in SLOT 3 , PCIe 8x(4,1)\|
		+	\|Dell Optiplex 990 \|Intel X38 \| PCIe 8x \| /* See ticket 828080*\

	===== not-working mainboards / computer =====		===== not-working mainboards / computer =====
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	^ Computer ^Chipset ^Comment^		^ Computer ^Chipset ^Comment^
	\|DELL Vostro 630 \| \| \|		\|DELL Vostro 630 \| \| \|
		+	\|DELL Optiplex 9010 \| \| \|
	\|DELL Optiplex 9020 Model Minitower \| \| \|		\|DELL Optiplex 9020 Model Minitower \| \| \|
	\|DELL Optiplex 7020 \| \| \|		\|DELL Optiplex 7020 \| \| \|
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	\|HP 200-G1 (Model L5L54US#ABA) \| \| \|		\|HP 200-G1 (Model L5L54US#ABA) \| \| \|
	\|Lenovo ThinkServer TS140 \| \| \|		\|Lenovo ThinkServer TS140 \| \| \|
		+
		+	*/

fcs

Anonymous (anonymous@undisclosed.example.com) — 2015-01-22T14:55:15+00:00

2026/04/22 23:39	current
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	+	====== Fluorescence Correlation Spectroscopy (FCS) ======
	+	Fluorescence correlation spectroscopy (FCS) is a correlation analysis of fluctuation of the fluorescence intensity. The analysis provides parameters of the physics under the fluctuations. One of the interesting applications of this is an analysis of the concentration fluctuations of fluorescent particles (molecules) in solution. In this application, the fluorescence emitted from a very tiny space in solution containing a small number of fluorescent particles (molecules) is observed. The fluorescence intensity is fluctuating due to Brownian motion of the particles. In other words, the number of the particles in the sub-space defined by the optical system is randomly changing around the average number. The analysis gives the average number of fluorescent particles and average diffusion time, when the particle is passing through the space. Eventually, both the concentration and size of the particle (molecule) are determined. Both parameters are important in biochemical research, biophysics, and chemistry.(([[http://en.wikipedia.org/wiki/Fluorescence_correlation_spectroscopy]]))

	+
	+	see [[general:fluorescence_correlation_spectroscopy-_a_short_introduction\|Introduction to Fluorescence Correlation Spectroscopy]].

marquardt-levenberg

Anonymous (anonymous@undisclosed.example.com) — 2015-02-10T16:34:33+00:00

2014/01/21 16:37		current
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	====== Marquardt-Levenberg ======		====== Marquardt-Levenberg ======

-	The Marquardt-Levenberg method is a means of optimising parameters in a least squares fit. It is used in our [[FluoFit]], [[SymPhoTime]] and a variety of other products (online-Fitting, FluoLib...).	+	The Marquardt-Levenberg method is a means of optimising parameters in a least squares fit. It is used in our [[software:FluoFit]], [[software:SymPhoTime]] and a variety of other products (online-Fitting, FluoLib...).

	Basically it works by examining how changes of a certain parameter affect the residuals trace, that means, it calculates a pointwise gradient of the residuals trace for each parameter. Marquard-Levenberg optimisation is fairly efficient and is regarded as state-of-the-art in least squares fitting.		Basically it works by examining how changes of a certain parameter affect the residuals trace, that means, it calculates a pointwise gradient of the residuals trace for each parameter. Marquard-Levenberg optimisation is fairly efficient and is regarded as state-of-the-art in least squares fitting.

pattern_matching

Anonymous (anonymous@undisclosed.example.com) — 2014-06-18T13:26:07+00:00

2014/05/06 14:41		current
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-	{{tag> howto imaging FLIM pattern-matching tutorial analysis}}	+	{{tag> howto imaging FLIM pattern-matching tutorial analysis SymPhoTime}}

	~~NOTOC~~		~~NOTOC~~
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	Pattern Matching Analysis by decomposing a recorded image into different user-defined patterns. Display of the calculated data using an RGB false color model. The calculated amplitudes of the first three defined patterns are depicted in three different colors for each pixel.		Pattern Matching Analysis by decomposing a recorded image into different user-defined patterns. Display of the calculated data using an RGB false color model. The calculated amplitudes of the first three defined patterns are depicted in three different colors for each pixel.
		+
		+	{{ youtube>L68IgRbNSLE?large }}

	==== Open an Image ====		==== Open an Image ====

tcspc_external_markers

Anonymous (anonymous@undisclosed.example.com) — 2020-09-14T16:01:28+00:00

2019/07/09 13:39		current
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	* PI Controller: E725		* PI Controller: E725
	* PI Controller: E710 \\		* PI Controller: E710 \\
		+	* PI Controller: E727 \\
		+	* Controller: C867.2U2 (PI wide range scanner)
		+	* Controller: C867.262 (PI wide range scanner)
		+
	which have been used in PicoQuant systems before [[http://tcspc.com/doku.php/software:supported_mt200_pc_configuration_for_symphotime_64\|and also MadCity labs can help you getting the proper cable for their stages. ]]		which have been used in PicoQuant systems before [[http://tcspc.com/doku.php/software:supported_mt200_pc_configuration_for_symphotime_64\|and also MadCity labs can help you getting the proper cable for their stages. ]]
		+
		+

2020/04/14 11:40		current
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	===== News from the community=====		===== News from the community=====
		+	* 29.04.20 [[https://www.fret.community/announcement-protein-folding-and-dynamics-webinar/\|Protein folding and dynamics Webinar-Series.]] \\ \\
	* 14.04.20 [[https://analyticalscience.wiley.com/do/10.1002/was.00050003/full/bioimagedataanalysis.pdf\|Bioimage Data Analysis.]] New Expanded version of a nice open-source introduction. \\ \\		* 14.04.20 [[https://analyticalscience.wiley.com/do/10.1002/was.00050003/full/bioimagedataanalysis.pdf\|Bioimage Data Analysis.]] New Expanded version of a nice open-source introduction. \\ \\
-	* 14.04.20 [[https://science.sciencemag.org/content/368/6486/78\|De novo design of protein logic gates.]] Fundamendal enzymology work. Chen et al. describe the design of logic gates that can regulate protein association. The gates were built from small, designed proteins that all have a similar structure but where one module can be designed to interact specifically with another module. Using monomers and covalently connected monomers as inputs and encoding specificity through designed hydrogen-bond networks allowed the construction of two-input or three-input gates based on competitive binding. \\ \\	+	* 14.04.20 [[https://science.sciencemag.org/content/368/6486/78\|De novo design of protein logic gates.]] Fundamental enzymology work. Chen et al. describe the design of logic gates that can regulate protein association. The gates were built from small, designed proteins that all have a similar structure but where one module can be designed to interact specifically with another module. Using monomers and covalently connected monomers as inputs and encoding specificity through designed hydrogen-bond networks allowed the construction of two-input or three-input gates based on competitive binding. \\ \\
	* 31.03.20 FRET community has a new advisory board, [[https://www.fret.community/\|FRET community]]. Happy that Dr. Marcelle König , a senior scientist in PicoQuant was elected. \\ \\		* 31.03.20 FRET community has a new advisory board, [[https://www.fret.community/\|FRET community]]. Happy that Dr. Marcelle König , a senior scientist in PicoQuant was elected. \\ \\
	* 31.03.20 Extremely helpful reference paper about confocal microscopy. [[https://www.nature.com/articles/s41596-020-0313-9\|Tutorial: guidance for quantitative confocal microscopy]]. Available as a poster [[https://www.nature.com/articles/s41596-020-0307-7\|here.]] \\ \\		* 31.03.20 Extremely helpful reference paper about confocal microscopy. [[https://www.nature.com/articles/s41596-020-0313-9\|Tutorial: guidance for quantitative confocal microscopy]]. Available as a poster [[https://www.nature.com/articles/s41596-020-0307-7\|here.]] \\ \\