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--- video_tutorials [2014/05/06 11:41] – admin
+++ video_tutorials [2014/06/18 09:58] – admin
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 Fluorescence and Fluorescence Microscopy explained by [[http://www.ibiology.org/ibioseminars/nico-stuurman-biography.html|Nico Stuurman]] at UCSF.
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 [[http://www.ibiology.org/ibioseminars/techniques/nico-stuurman-part-1.html]]
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 ==== Confocal Microscopy ====
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 Nikons website [[http://www.microscopyu.com/articles/confocal/index.html|MicroscopyU.com]] has a very nice course on confocal microscopy.
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 Confocal Microscopy explained by Kurt Thorn of the Nikon imaging center at UCSF
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 [[http://www.ibiology.org/ibioseminars/techniques/kurt-thorn-part-1.html]]
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 Kurt Thorn of UCSF talks about two-photon microscopy which uses intense pulsed lasers to image deep into biological samples. It can be used for imaging thick tissue specimens or even imaging inside of live animals.
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 [[http://www.ibiology.org/ibioeducation/taking-courses/two-photon-microscopy.html]]
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 The lifetime of the fluorophore signal, rather than its intensity, is used to create the image in FLIM. This has the advantage of minimizing the effect of photon scattering in thick layers of sample ([[wp>Fluorescence-lifetime_imaging_microscopy | FLIM]]).
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 [[http://www.ibiology.org/ibioeducation/taking-courses/fluorescence-lifetime-imaging-microscopy.html]]