xample image used is an image showing four cells, two of them only transfected with the CENP-B-Cerulean complex, the other two transfected with both constructs. Therefore, the ... ted.
**Note:**\\
Now four cells are visible, the two nuclei on the left have longer Fast lifetimes then the other two. These are the cells transfected only with the do
as for fluorescence measurement
</code>
==== Two photon excitation (TPE) ====
Do not attempt to r... ysis of time domain measurements =====
There are two major ways of compensating IRF effects:
*cor
Image_5.png?600 }}
**Note:** The window contains two different regions:\\
- **Left:** Fitting and an... splayed in the script. For illustration, mark the two files ''Cy5_diff_IRF+FLCS-pattern.ptu'' and ''ATT... . In our example, it is of course meaningless, as two different dyes were fitted.
* Place the mouse