{{tag> pile-up dead_time IRF differential_countrate}}
====== Differential Count Rate ======
Ideally, a... y (probability of encountering a photon) during a time interval when a detected signal is really present... the detector bunched, as flashes. These are short time intervals with huge photon density (rate), separa... rate" is a result of **sparse sampling with dead time**. The sampled signal features much higher photon
may trigger the avalanche process and generates a time-corresponding electrical pulse. The avalanche mus... just a resistor but is slow and causes longer deadtimes. Active quenching is standard for all modules us
FLIM ======
[[FLIM]] image, using the [[fast lifetime]] calculation. The color-coded fast lifetime of each pixel represents the average photon arrival time with respect to the preceding laser pulse.
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FLIM stands for **F**luorescence **L**ifetime **IM**aging or **F**luorescence **LI**fetime **M**icroscopy. ([[wp>Fluorescence-lifetime imaging microscopy]]).
In FLIM additional image contrast t... used to visualize the average [[fluorescence lifetime]] in each pixel of the image.
===== FLIM on this
{{tag> TCSPC pile-up dead_time}}
~~TOC~~
====== Pile-Up Effect ======
The Pile-Up effect describes the ... s lost at high photon count rates due to the dead time of the TCSPC devices.
Most single photon counti... detectors and [[TCSPC]] electronics have a [[dead time]]. After registering one photon the device needs some time to be ready for the next photon to detect. The de
====== Dead Time ======
In TCSPC the term dead time refers to the time the TCSPC system needs armed again after detecting an event. During the dead time the TCSPC system is blind.
If for example two p
====== TTTR ======
TTTR stands for Time-Tagged Time-Resolved. The basic concept of this measurement mode is to record every individual photon of a [[TCSPC]] experiment. Both the arrival time as well as the TCSPC timing information (i.e. the time from the preceding excitation pulse or to the fol
== Reconvolution ======
Reconvolution is used in time domain data analysis for removal of broadening ef... ftware packages (mainly [[software:FluoFit]] and [[software:SymPhoTime]]) for compensating IRF effects.
sed in our [[software:FluoFit]], [[software:SymPhoTime]] and a variety of other products (online-Fitting... traight-forward manner). Especially for the SymPhoTime software this is a severe limitation, since least... in [[FLIM]] measurements as analysed by the SymPhoTime software the number of photons for a given decay ... ]] fitting.
When running a [[MLE]] fit the SymPhoTime software abandons the ML-fitting and switches to
**B**urst **I**ntegrated **F**luorescence **L**ifetime: The photon events of a complete burst are accumu... into a [[TCSPC]] histogram which is used for lifetime analysis methods afterwards. Usually a burst is defined as a time interval for which the intensity of a time trace or [[MCS]] measurement is above a chosen threshold. Ev