rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... background is usually very long (up to 1/2 of the TCSPC window), which makes the color scale loaded by de... g is then applied to the lifetime graph(s) in the TCSPC histogram.\\
**Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (=
are the maintainer of a software that deals with TCSPC data please edit this page and add your software ... me Correlated Single Photon Counting Instruments (TCSPC devices). It harnesses the advantages of C++ for ... een the high-speed capabilities of your PicoQuant TCSPC device and the ease of use and versatility of Pyt... o analyze time-correlated single-photon counting (TCSPC) data sets, namely fluorescence lifetime and time
ixel resolution in μm |
|TCSPCChannels||||''int32'' |number of TCSPC channels per pixel |
|TimeResol ... ||||''float32'' |time resolution of the TCSPC histograms in ns |
|The following block... ll appear in the block (x,y) for each ''t = 1 to <TCSPCChannels>''||||
| | | | ''HistogramData [x,y,t]''
lysis" tab and in there, open the drop down menu "TCSPC".
{{ lifetime_fitting_using_the_tcpsc_fitting_scr... op down menu: {{grey_button.png}}
* Start the "TCSPC Fitting" script by clicking on "Start".
{{ lifeti... _fitting_script_Image_4.png }}
**Response:** The TCSPC Fitting script is applied to the file ''ATTO655_d... **Center/right:** TCPSC fitting graph showing the TCSPC histogram of the measured sample.
* In the de
rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\
**5: [[glossary:TCSPC]] Decay window**\\
By default, this set shows the [[glossary:TCSP
rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... background is usually very long (up to 1/2 of the TCSPC window), which makes the color scale loaded by de... ied to the lifetime graph(s) on the lower center (TCSPC histogram).\\
- **Lower center/left:** By default, this set shows the TCSPC histogram of all photons in the image in green (=
rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\
**5: [[glossary:TCSPC]] Decay window**\\
By default, this set shows the [[glossary:TCSP
ted Hardware (for MicroTime200):=====
====Harps (TCSPC units)====
===PicoHarp 300===
* For imaging, o... * If your system is (to be) equipped with a TH260 TCSPC card this card as also to be installed into a PCI
re possible which are described here: https://www.tcspc.com/doku.php/howto:how_to_measure_the_instrument_... has been stopped, when the decay histogram in the TCSPC preview has reached ~10000 counts in the peak cha... 57fcfa684daef86c23]].\\
2. In Analysis tab, under TCSPC, find `TCSPC Decay`. Click Start;
{{ howto:tcspc_decay_gui.png?400 |}}
Response: you see this kind of c
rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\
**5: [[glossary:TCSPC]] Decay window**\\
By default, this set shows the [[glossary:TCSP
rescence is detected, is randomly spread over the TCSPC window, the mean photon arrival time of the dark ... ually very long (with an average up to 1/2 of the TCSPC window), which makes the color scale loaded by de... lifetime graph(s) on the lower center ([[glossary:TCSPC]] histogram).\\
**5: [[glossary:TCSPC]] Decay window**\\
By default, this set shows the [[glossary:TCSP
odules could be Laser Drivers, Piezo Controllers, TCSPC modules, PCAN controller card, Framegrabber card ... h Microtime and LSM upgrade kits Users====
* **TCSPC **: Use the drop down menu to select the proper TCSPC module then click "Init.TCSPC".
* **Light-Source driver**: Use the drop down menu to selct the proper
ing with Time Resolved Fluorescence or [[glossary:TCSPC]] in general. It is not meant to be complete.
==... stallation |Configuring SymPhoTime64]]
[[support:tcspc_external_markers|TCSPC Modules and External Markers]]
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