e file ''CENP-labelled _cells_for_FRET.ptu'' by a single mouse click.
{{ flim-fret-calculation_for_multi-e... green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red an estimation... ''CENP-labelled _cells_for_FRET.ptu'' again by a single mouse click.
{{ flim-fret-calculation_for_multi-e... can only be calculated if the FRET donor dye has single exponential decay kinetics, changing the fitting
ghlight the file ''Kidney_Cells_FLIM.ptu'' by a single mouse click.
* Select the **"Analysis"** tab ... aset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red an estimation... oexpo_roi0_kidney_3.png |}}
** Response:**\\
A single exponential reconvolution fit is performed. In th... * In this example it is obvious that the applied single exponential reconvolution fit is not suited to fi
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*Start Point Measurement
*For a single immobilized molecule antibunching trace you will ... u P., Ko H. C., Huang J., Tang J.: //Antibunching single-photon emission and blinking suppression of CdSe/... B: //Ultrafast dynamics of protein collapse from single-molecule photon statistics//. In: //Proceedings o
e lifetime of a measured sample. As an example, a single exponential reconvolution fit is used to determin... t the file ''ATTO655_diff_FLCS-pattern.ptu'' by a single mouse click.
{{ lifetime_fitting_using_the_tcpsc_... ".
**Response:** Both data sets are fitted with a single exponential tailfit model. The values of the last