PhoTime64|SymPhoTime 64]] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** The "Samples" workspace is delivered with the SymPhoTime 64 an... bjective, but can also be caused by degraded test samples or too high laser power, which leads to photoblea
ducts:SymPhoTime64|SymPhoTime 64]].
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:**
The "Samples" workspace is delivered with the [[products:SymPh... ide of the main window.
{{ :howto:flim-fret-1expd_samples_ws.png |}}
* Highlight the file ''FRET_GFP and
sis is started using a daisy pollen image in the "Samples" workspace, a suited fitting model is chosen and ... =
* Start SymPhoTime 64 software
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:**\\
The "Samples" workspace is delivered with the SymPhoTime 64 an
t is started using a daisy pollen image from the "Samples" – workspace, then three regions of interest are ... PhoTime64|SymPhoTime 64]] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** The "Samples" workspace is delivered with the SymPhoTime 64 an
icantly larger. Therefore, to measure immobilized samples usually pulsed excitation is used.
===== Step-by... PhoTime64|SymPhoTime 64]] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** The "Samples" workspace is delivered with the SymPhoTime 64 an
in front of the tube lens.}}]
*//diffusing Samples:// typically just point measurement in [[glossary:T2-mode]] (see below)
*//immobilized samples:// imaging in [[glossary:T3-mode]] followed by po
PhoTime64|SymPhoTime 64]] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
* The "Samples" workspace is delivered with the SymPhoTime 64 on
oTime64|SymPhoTime 64]] software.\\
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** The "Samples" workspace is delivered with the SymPhoTime 64 an
PhoTime64|SymPhoTime 64]] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** The "Samples" workspace is delivered with the SymPhoTime 64 an
PhoTime64|SymPhoTime 64]] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** The "Samples" workspace is delivered with the SymPhoTime 64 on
PhoTime64|SymPhoTime 64]] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** The "Samples" workspace is delivered with the SymPhoTime 64 an
PhoTime64|SymPhoTime 64]] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** The "Samples" workspace is delivered with the SymPhoTime 64 an
the NV-ceneters make for excellent demonstration samples for [[antibunching measurements]]. They are also ... ability makes the nv-centers ideal single emitter samples compared to single molecules the laser power to e
* Start SymPhoTime 64 software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:**\\
The "Samples" workspace is delivered with the SymPhoTime 64 an