====== Advantages and disadvantages of two photon excitation (TPE) ======
*The main advantage of two photon excitation is a deeper penetration and less photo...
*The absorption is much less compared to one photon excitation. This leads very often to high intensi... al.
===== Laser Safety =====
Lasers used for 2 Photon Excitation are usually laser class IV. Laser Clas
S0-S1 excitation) and cannot emit a fluorescence photon.
{{:general:singlet-triplet-fluctuations.png|}}
... tion spectroscopy by using time-correlated single photon counting. \\ Triffo et al, 2012, "Monitoring Lipi... s of proteome dynamics in living cells"
* **two-photon FCS** \\ Schwille and Heinze, 2001, "Two-Photon cross-correlation spectroscopy"
* **scanning FCS** \\ R