mage_9.png }}
**Note:**\\
For more information about the different ROI selection tools, check the tuto... th donor and acceptor construct and two cells without. For the cells with the FRET donor only, a FRET e... nce requires some effort and distinct knowledge about the sample. A few aids are available on the lab.
s, and it is very likely to produce artefacts without allowing to tell apart artefacts and effects.
==... lso a remarkably short fluorescence lifetime of about 10 ps. This short lifetime does not depend on the
ors directly to the [[Products:PicoHarp 300]] without passing SPAD 2 through the router. **In this case... displayed.
====== Some MT200 based literature about Antibunching ======
-Yuan C. T., Yu P., Ko H
h a TiSa-laser with 80MHz repetition rate). Check out the rapidReconvolution tutorial for this case (ht... d_reconvolution_algorithm).\\
For explanations about the fitting model and the used equations, click o... **\\
For pixel-by-pixel fits, we are interested about how the lifetime parameters are distributed in th
ry selection, the corresponding red dot is greyed out.
{{ roi_fitting_using_the_flim_script_Image_14.pn... image are highlighted, everything else is greyed out. The decay corresponding to ROI 2 contains now th... e is usually a bit arbitrary.\\
For explanation about the fitting model and the used equations, click o
scribed here can also be used for IRF-fitting without time gating. However, as these decays contain the... ly. Please check the different FLIM-related tutorials for more information about the fitting.
is usually a bit arbitrary.\\
For explanation about the fitting model and the used equations, click o... er adding the sugar, has been dropped gradually about 500ps.\\
* If an IRF has been measured, the fi... hown in the SymPhoTime 64 window, e.g with or without scale bar (depending on whether you have the scal
ontains manually entered text (e.g. information about the experiment)|Menu: **File → Create Comment**|D... ns a file viewer, in which you find information about the file type and acquisition settings
<imgcapti... line-analysis will contain relevant information about the measurement. To check the values calculated d... in before the acquisition (see online tutorials about FLIM and FLIM-FRET imaging)
===== Working wit
_at_14-35-37.jpg?600 |}}
* Select either IN or OUT position
**Note:** The sample holder has two inde... on beam will directly hit the sample, when in the OUT position the sample will only be illuminated indi... der into the IN-position.
* Select either IN or OUT position.
**Note:** The position should be the s
ion is used to distinguish molecules with and without acceptor dyes and to filter the events for signals without acceptor to calculate the stoichiometry parameter... n intensity threshold is calculated for filtering out events without acceptor present. Four the fourth trace, also an intensity threshold is calculated, whi
not necessary to calculate first a FCS curve without the photon filter. You can also directly activate... the FCS Calibration Script]] for a description
about how to calibrate the confocal volume.\\
If no cal... the fitting parameter. If you
need information about how the fitting models are defined and what is th