Ch.0.
-Disconnect SPAD 1 Signal from PHR800 Router.
-Connect SPAD 1 Signal to [[Products:Pico... is NIM compatible. If the SPAD provides only TTL output pulses, you need to use the SIA400 adapter.
... ors directly to the [[Products:PicoHarp 300]] without passing SPAD 2 through the router. **In this case, however, the router cable (DVI-cable) needs to be d
cy using the "Lifetime FRET Image" analysis.
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===== Background informa... mage_9.png }}
**Note:**\\
For more information about the different ROI selection tools, check the tuto... of importance. Therefore exclude the bright dots outside the nucleus of the cell on the lower right by... ously "CRTL" and draw rectangles around the spots outside the nucleus.
* **Response:** The marked reg
ow to measure na IRF on confocal microscopes.
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\\
==== Low count rate ... s, and it is very likely to produce artefacts without allowing to tell apart artefacts and effects.
==... lso a remarkably short fluorescence lifetime of about 10 ps. This short lifetime does not depend on the
ne the lifetime of ATTO655 diluted in water.
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===== Step-by-Step Tutor... e is usually a bit arbitrary.\\
For explanation about the fitting model and the used equations, click o
h a TiSa-laser with 80MHz repetition rate). Check out the rapidReconvolution tutorial for this case (ht... d_reconvolution_algorithm).\\
For explanations about the fitting model and the used equations, click o... **\\
For pixel-by-pixel fits, we are interested about how the lifetime parameters are distributed in th