the detection volume. As molecule diffuse in and out the relative fluctuations of the fluorescent inte... sity are large (no intensity when the molecule is out, high intensity when the molecule is in) in compa... at the fluctuations due to molecules going in and out of the volume will be smaller than the noise inhe
_at_14-35-37.jpg?600 |}}
* Select either IN or OUT position
**Note:** The sample holder has two inde... on beam will directly hit the sample, when in the OUT position the sample will only be illuminated indi... der into the IN-position.
* Select either IN or OUT position.
**Note:** The position should be the s
ry selection, the corresponding red dot is greyed out.
{{ roi_fitting_using_the_flim_script_Image_14.pn... image are highlighted, everything else is greyed out. The decay corresponding to ROI 2 contains now th