nt the correlation curve will become flat.
==== Objective Types ====
An excellent reference for the subject... 10 μm inside the sample. While measuring with oil objective one observes a very strong dependence of the shap... er practical issue is the working distance of the objective in combination with the refractive index mismatch... ndex mismatch between the immersion medium of the objective and the measurement medium and also if the medium
ion is necessary as well as a high N.A.
*used objective: N.A. 1.3 is working, better 1.45 oil!
*Altho... ments were possible also with the water immersion objective: with 60x1.2 water less than 50% count rate in comparison to 100x1.3 oil
*0.95 N.A. air objective did not yield any usable results.
*excitation... 2 bandpasses directly in front of detectors)
*objective: 1.3 N.A. oil immersion
[{{ howto:emission_nv-c
hole diameter divided by the magnification of the objective.
e.g:
*60x 1.2N.A. Objective, 640nm excitation
*excitation FWHM = 350 nm (typical for 4x out-coupl... nm $
Together with the 60x magnification of the objective the airy disc at the pinhole location is
$ d_{PH... nm $
Together with the 60x magnification of the objective both airy discs together, at the the pinhole loca
ope with UPlanSApo 60x, NA = 1.2, water immersion objective.)
This is indeed normal, it is the result of u... y position the beam to enter at the center of the objective entrance pupil, and the beam must be of course aligned with the optical axis of the objective. (Note that parallel beam displacement, not just
.A.) * M$$
where $M$ is the magnification of the objective
e.g.:
* 60x water immersion Olympus objective, NA 1.2
* Dye: Alexa 430 (em. max: 539 nm).
=> $d_{Airy}