==== Software ======
===== Commercial =====
==== Microscopy ====
[[software:SymPhoTime64]] \\
A complete s... eractive phasor analysis of Fluorescence Lifetime Microscopy (FLIM) data [[https://github.com/LaboratoryOptics... package for quantitative analysis of fluorescence microscopy and spectroscopy data, with a focus on experiment... /github.com/tsbischof/libpicoquant]]
* **Open Microscopy's BioFormat** supports PicoQuant bin files ([[glo
ly measured before or after each measurement.
In microscopy-applications, this is usually not practical; and ... recommend to measure the IRF as scatters light in microscopy, due to the color dependence of SPAD detectors, which are generally used in microscopy. Furthermore, even with detectors lacking color e
{{tag> basics introduction FCS fluorescence microscopy objective resolution NA tutorials}}
===== Basics =====
... * [[video_tutorials|Fluorescence and Fluorescence Microscopy by Nico Stuurman]]
* [[video_tutorials#Kurt Thorn: Optical Sectioning and Confocal Microscopy|Confocal Microscopy and Optical Sectioning by Kurt Thorn]]
* For a complete Video based Microscopy course
Extremely helpful reference paper about confocal microscopy. [[https://www.nature.com/articles/s41596-020-0313-9|Tutorial: guidance for quantitative confocal microscopy]]. Available as a poster [[https://www.nature.com... 1.20** Exciting work combining FLIM-FRET and STED microscopy in vivo using a newly developed FRET pair! This c... one as revealed by correlative FLIM-FRET and STED microscopy]] \\ \\
* **24.10.19** New publication [[https
of multiexponetial decays for pure dyes]]
===== Microscopy =====
[[http://www.picoquant.com/images/uploads/... es.pdf|Quick Reference for Confocal Time-resolved Microscopy (FLIM, FRET, FCS)]] - Poster (pdf format)
[[video_tutorials|Fluorescence and fluorescence microscopy by Nico Stuurman]]
[[video_tutorials#Kurt Thorn: Optical Sectioning and Confocal Microscopy|Confocal microscoy and optical sectioning by Kurt