lsm710
This is an old revision of the document!
FLIM Measurement Using a Zeiss LSM710 with a FLIM and FCS Upgrade
This tutorial shows the recording of FLIM images using an LSM upgrade kit, in this case a Zeiss LSM710. In FLIM-measurements, the fluorescence lifetime of a component can be determined which can be used e.g.
- to distinguish different tissue or cell constituents
- to monitor concentration changes using a reporter dye
- To monitor interactions between two molecules via FRET.
The data acquisition is general principles of the data acquisition are analogous in all LSM upgrade kits.
Continue to
lsm710.1469016068.txt.gz · Last modified: 2016/07/20 12:01 by buschmann