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| lifetime_component_decomposition_phasor_plot_analysis [2025/01/15 09:46] – lan | lifetime_component_decomposition_phasor_plot_analysis [2025/06/10 10:35] (current) – lan | ||
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| ====== LSM Upgrade Kit on Nikon AX: Lifetime Component Decomposition in Phasor Plot ====== | ====== LSM Upgrade Kit on Nikon AX: Lifetime Component Decomposition in Phasor Plot ====== | ||
| + | * Software information: | ||
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| + | * Software information: | ||
| {{ :Lifetime Component Decomposition in Plasor Plot.mp4? | {{ :Lifetime Component Decomposition in Plasor Plot.mp4? | ||
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| **Introduction** | **Introduction** | ||
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| In the Nikon AX-PicoQuant FLIM system, using a single spectral window enables the differentiation of fluorescent species or environments solely based on their lifetimes. This method simplifies both the experimental setup and data analysis by eliminating the need for multiple spectral channels while still providing precise identification of distinct lifetime populations. This streamlined approach enhances the efficiency of resolving lifetime components in complex biological systems. | In the Nikon AX-PicoQuant FLIM system, using a single spectral window enables the differentiation of fluorescent species or environments solely based on their lifetimes. This method simplifies both the experimental setup and data analysis by eliminating the need for multiple spectral channels while still providing precise identification of distinct lifetime populations. This streamlined approach enhances the efficiency of resolving lifetime components in complex biological systems. | ||
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| - | **Software Information** | ||
| - | * NIS Element version 5.42.06 with PicoQuant FLIM-FCS full integration | ||
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| **Sample Information** | **Sample Information** | ||