howto:visualizing_dynamics_with_the_multi_frame_flim_analysis
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howto:visualizing_dynamics_with_the_multi_frame_flim_analysis [2015/11/03 14:31] – admin | howto:visualizing_dynamics_with_the_multi_frame_flim_analysis [2015/11/03 14:32] – admin | ||
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**Note:** The window contains five different regions:\\ | **Note:** The window contains five different regions:\\ | ||
- **Upper left:** Imaging analysis options. As there are many options in this panel, not all of them fit into the visible area of the screen. Thus, a scroll bar is generated to access all controls.\\ | - **Upper left:** Imaging analysis options. As there are many options in this panel, not all of them fit into the visible area of the screen. Thus, a scroll bar is generated to access all controls.\\ | ||
- | - **Upper center:** [[glossary: | + | - **Upper center:** [[glossary: |
- **Upper right:** Fluorescence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifetimes is plotted. The settings of the plot can be adapted using the controls on the right of the plot.\\ | - **Upper right:** Fluorescence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifetimes is plotted. The settings of the plot can be adapted using the controls on the right of the plot.\\ | ||
- | - **Lower left:** Lifetime fitting parameters allowing to define the lifetime fitting models. Fitting is then applied to the lifetime graph(s) in the TCSPC histogram.\\ | + | - **Lower left:** Lifetime fitting parameters allowing to define the lifetime fitting models. Fitting is then applied to the lifetime graph(s) in the [[glossary:TCSPC]] histogram.\\ |
- | - **Lower center/ | + | - **Lower center/ |
* To enhance the lifetime contrast within the image, adapt the color scale from 0 to 15 ns by typing these values as limits for the Fast Lifetime color scale. Also adjust the intensity scale to 1 – 200 counts. | * To enhance the lifetime contrast within the image, adapt the color scale from 0 to 15 ns by typing these values as limits for the Fast Lifetime color scale. Also adjust the intensity scale to 1 – 200 counts. |
howto/visualizing_dynamics_with_the_multi_frame_flim_analysis.txt · Last modified: 2020/05/13 09:39 by ruckelshausen