Differences

This shows you the differences between two versions of the page.

--- howto:visualizing_dynamics_using_the_multiframe-flim_script [2020/05/13 09:39] – [Visualizing Dynamics using the Multi Frame FLIM Analysis (SPT64 V2.5)] ruckelshausen
+++ howto:visualizing_dynamics_using_the_multiframe-flim_script [2021/10/19 19:59] – [Visualizing Dynamics Using the Multi Frame FLIM Analysis (updated for SymPhoTime v2.5)] rezvani
@@ Line 1: / Line 1: @@
 {{tag>howto FLIM multi-frame imaging analysis dynamics tutorial LSM_upgrade SPT SymPhoTime}}
-====== Visualizing Dynamics Using the Multi Frame FLIM Analysis (updated for SymPhoTime v2.5) ======
+====== Visualizing Dynamics Using the Multi Frame FLIM Analysis (updated for SymPhoTime v2.5 and above) ======
 ===== Introduction =====
 This tutorial shows step-by-step, how to separate the different frames of multi-frame image  with systems equipped with a fast Galvo scanner. The image containing many frames is treated here like a time-series. The example image shows the plasma membrane of MDCK cells stained with Flipper-TR, which can report membrane tension changes through its fluorescence lifetime changes. This has been published 2018 in nature:
-[[https://www.nature.com/articles/s41557-018-0127-3]]
+[[https://www.nature.com/articles/s41557-018-0127-3]].
 This dynamics is visualized with the "multi Frame FLIM" script. Only few of the frames are binned together for analysis, and an image series is obtained. The lifetime change will be monitored at the end.
+Tutorials workspace can be downloaded from [[https://figshare.com/s/4957fcfa684daef86c23]].
 ===== Step-by-Step Tutorial =====