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--- howto:using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs [2014/05/23 07:47] – admin
+++ howto:using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs [2015/02/25 14:00] (current) – bornemann
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 {{tag> howto tutorial FCS FLCS crosstalk analysis SymPhoTime}}
-~~TOC~~
+~~NOTOC~~
-====== Spectral crosstalk removal via FLCCS ======
+====== Spectral Crosstalk Removal via FLCCS ======
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 This tutorial shows step-by-step, how to calculate a spectral fluorescence lifetime cross-correlation (FLCCS) curve from a measurement of a mixture of two labeled oligunucleotides moving independently from each other. As the green dye has been excited with pulsed 485 nm excitation, while the red dye has been excited with a 560 nm cw laser, the different excitation scheme is used to eliminate the unwanted spectral crosstalk.
+{{ youtube>O2lTTF70tZE?large }}
 ===== Background Information =====
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 **Response:** The correlation curves of this file are calculated. The cross-correlation amplitude is significantly higher than for the negative calibration dye as processed before, but lower than the corresponding FCCS file obtained with the "Grouped FCS" script.
 {{ using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs_Image_18.png?600 }}