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howto:using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs [2014/05/23 07:47] – adminhowto:using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs [2014/06/19 08:09] – admin
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 {{tag> howto tutorial FCS FLCS crosstalk analysis SymPhoTime}} {{tag> howto tutorial FCS FLCS crosstalk analysis SymPhoTime}}
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 ====== Spectral crosstalk removal via FLCCS ====== ====== Spectral crosstalk removal via FLCCS ======
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 This tutorial shows step-by-step, how to calculate a spectral fluorescence lifetime cross-correlation (FLCCS) curve from a measurement of a mixture of two labeled oligunucleotides moving independently from each other. As the green dye has been excited with pulsed 485 nm excitation, while the red dye has been excited with a 560 nm cw laser, the different excitation scheme is used to eliminate the unwanted spectral crosstalk. This tutorial shows step-by-step, how to calculate a spectral fluorescence lifetime cross-correlation (FLCCS) curve from a measurement of a mixture of two labeled oligunucleotides moving independently from each other. As the green dye has been excited with pulsed 485 nm excitation, while the red dye has been excited with a 560 nm cw laser, the different excitation scheme is used to eliminate the unwanted spectral crosstalk.
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 ===== Background Information ===== ===== Background Information =====
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 **Response:** The correlation curves of this file are calculated. The cross-correlation amplitude is significantly higher than for the negative calibration dye as processed before, but lower than the corresponding FCCS file obtained with the "Grouped FCS" script. **Response:** The correlation curves of this file are calculated. The cross-correlation amplitude is significantly higher than for the negative calibration dye as processed before, but lower than the corresponding FCCS file obtained with the "Grouped FCS" script.
 {{ using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs_Image_18.png?600 }} {{ using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs_Image_18.png?600 }}
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howto/using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs.txt · Last modified: 2015/02/25 14:00 by bornemann