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howto:using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs [2014/05/23 07:47] – admin | howto:using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs [2014/06/19 08:09] – admin |
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This tutorial shows step-by-step, how to calculate a spectral fluorescence lifetime cross-correlation (FLCCS) curve from a measurement of a mixture of two labeled oligunucleotides moving independently from each other. As the green dye has been excited with pulsed 485 nm excitation, while the red dye has been excited with a 560 nm cw laser, the different excitation scheme is used to eliminate the unwanted spectral crosstalk. | This tutorial shows step-by-step, how to calculate a spectral fluorescence lifetime cross-correlation (FLCCS) curve from a measurement of a mixture of two labeled oligunucleotides moving independently from each other. As the green dye has been excited with pulsed 485 nm excitation, while the red dye has been excited with a 560 nm cw laser, the different excitation scheme is used to eliminate the unwanted spectral crosstalk. |
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===== Background Information ===== | ===== Background Information ===== |
**Response:** The correlation curves of this file are calculated. The cross-correlation amplitude is significantly higher than for the negative calibration dye as processed before, but lower than the corresponding FCCS file obtained with the "Grouped FCS" script. | **Response:** The correlation curves of this file are calculated. The cross-correlation amplitude is significantly higher than for the negative calibration dye as processed before, but lower than the corresponding FCCS file obtained with the "Grouped FCS" script. |
{{ using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs_Image_18.png?600 }} | {{ using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs_Image_18.png?600 }} |
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