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--- howto:using_the_anisotropy_image_script [2018/04/12 10:26] – [Select a file and start the script] doerr
+++ howto:using_the_anisotropy_image_script [2018/04/12 11:44] (current) – [Select a file and start the script] doerr
@@ Line 52: / Line 52: @@
 **Note:**
- The anisotropy values can theoretically spread between -0.5 and 1 for parallel or vertical excitation and emission dipoles respectively. The anisotropy value of 0 is expected in case of very fast rotation or extremely efficient energy transfer processes  (e.g. efficient  HOMO-FRET).  When measuring a randomly aligned ensemble due to photoselection the anisotropy varies with in the limits of  -0.2 and 0.4 instead of -0.5 and 1.
+ The anisotropy values can theoretically spread between -0.5 and 1 for perpendicular and parallel excitation and emission dipoles respectively. The anisotropy value of 0 is expected in case of very fast rotation or extremely efficient energy transfer processes  (e.g. efficient  HOMO-FRET).  When measuring a randomly aligned ensemble due to photoselection the anisotropy varies with in the limits of  -0.2 and 0.4 instead of -0.5 and 1.
 ==== Adapt the script to the image and the system parameters ====