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--- howto:using_the_anisotropy_image_script [2018/04/12 09:32] – [Select a file and start the script] doerr
+++ howto:using_the_anisotropy_image_script [2018/04/12 09:39] – [Select a file and start the script] doerr
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   - **Left:** Imaging analysis and processing options.
   - **Upper center:** Fluorescence intensity image, displayed in false color scale. The perpendicular and parallel  fluorescence intensities measured with the two detectors are displayed in green (perpendicular) and red (parallel).
-  - **Upper right:** Fluorescence anisotropy image. By default, the rainbow color scale is spread between -0.4 and 1, which approximately corresponds to the possible distributions for single molecules and aligned molecules (-0.5 and 1. When measuring a randomly aligned ensemble with parallel excitation and emission dipole moments, the anisotropy values can theoretically spread between 0 (very fast rotation or extremely efficient HOMO-FRET) and 0.4 (rotation significantly slower than the fluorescence lifetime, no HOMO-FRET).
+  - **Upper right:** Fluorescence anisotropy image. By default, the rainbow color scale is spread between -0.4 and 1 (for single molecules and aligned molecules the anisotropy can vary between -0.5 and 1). When measuring a randomly aligned ensemble with parallel excitation and emission dipole moments, the anisotropy values can theoretically spread between 0 (very fast rotation or extremely efficient HOMO-FRET) and 0.4 (rotation significantly slower than the fluorescence lifetime, no HOMO-FRET).
   - **Lower graph:** Anisotropy histogram over the different pixels.