howto:separation_of_2_species_with
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howto:separation_of_2_species_with [2014/05/23 07:45] – admin | howto:separation_of_2_species_with [2014/06/18 10:57] – admin | ||
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- | ====== Separation of 2 Species with Different Lifetimes Using the FLCS Analysis | + | ====== Separation of 2 Species with Different Lifetimes Using FLCS ====== |
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This tutorial shows step-by-step, | This tutorial shows step-by-step, | ||
- | ===== Background Information ===== | ||
- | **Note:**\\ | + | ===== Background Information ===== |
The two fluorophores ATTO655 (ATTO-Tec, Germany) and Cy5 (GE Healthcare) have almost identical spectral properties and are thus excited with the same wavelength (e.g. 635 nm) and detected at the same spectral range. As also their molecular weight is comparable, the diffusion constant is too similar to distinguish between both dyes in a mixture by means of standard FCS.\\ | The two fluorophores ATTO655 (ATTO-Tec, Germany) and Cy5 (GE Healthcare) have almost identical spectral properties and are thus excited with the same wavelength (e.g. 635 nm) and detected at the same spectral range. As also their molecular weight is comparable, the diffusion constant is too similar to distinguish between both dyes in a mixture by means of standard FCS.\\ | ||
Nevertheless, | Nevertheless, |
howto/separation_of_2_species_with.txt · Last modified: 2014/06/18 12:22 by admin