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howto:roi_fitting_using_the_flim_script [2014/06/18 15:42] adminhowto:roi_fitting_using_the_flim_script [2015/02/25 13:58] bornemann
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 ~~NOTOC~~ ~~NOTOC~~
  
-====== FLIM ROI fitting ======+====== FLIM ROI Fitting ======
  
  
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 {{ youtube>g6uyvLQW4nY?large }} {{ youtube>g6uyvLQW4nY?large }}
  
-==== Step-by-Step Tutorial ====+===== Step-by-Step =====
  
  
-=== Select a file and start the script ===+==== Select a file and start the script ====
  
   * Start [[products:SymPhoTime64|SymPhoTime 64]] software.   * Start [[products:SymPhoTime64|SymPhoTime 64]] software.
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 **Response:** The color scale in the image adapts and lifetime contrasts in the pollen become visible. **Response:** The color scale in the image adapts and lifetime contrasts in the pollen become visible.
  
-=== Select 3 Regions of Interest (ROI) ===+==== Select 3 Regions of Interest (ROI) ====
  
  
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 **ASCII** exports the image as ASCII array using a text file format. Thereby one array encodes the intensity values, while a second array encodes the Fast Lifetime values. After removal of the header and the unwanted array, individual arrays can be imported in graphic programs, e.g. ImageJ as a text image. These images contain the full count resolution of the raw image.  **ASCII** exports the image as ASCII array using a text file format. Thereby one array encodes the intensity values, while a second array encodes the Fast Lifetime values. After removal of the header and the unwanted array, individual arrays can be imported in graphic programs, e.g. ImageJ as a text image. These images contain the full count resolution of the raw image. 
  
-=== Perform a Lifetime Fit on the Selected Regions (ROIs) ===+==== Perform a Lifetime Fit on the Selected Regions (ROIs) ====
  
   * In the decay form on the lower left, select n-exponential reconvolution as fitting model.   * In the decay form on the lower left, select n-exponential reconvolution as fitting model.
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 {{ roi_fitting_using_the_flim_script_Image_25.png?600 }} {{ roi_fitting_using_the_flim_script_Image_25.png?600 }}
  
-**Respond:** A window opens, asking for a file name to store the fitting values. The data are stored as a .dat file.+**Response:** A window opens, asking for a file name to store the fitting values. The data are stored as a ''.dat'' file.
 {{ roi_fitting_using_the_flim_script_Image_26.png?600 }} {{ roi_fitting_using_the_flim_script_Image_26.png?600 }}
  
-  * The .dat file contains the fitting values as table and can be opened e.g. using the Notepad program. They can also be imported into Excel or similar programs.+  * The ''.dat'' file contains the fitting values as table and can be opened e.g. using the Notepad program. They can also be imported into Excel or similar programs.
 {{ roi_fitting_using_the_flim_script_Image_27.png?600 }} {{ roi_fitting_using_the_flim_script_Image_27.png?600 }}
  
howto/roi_fitting_using_the_flim_script.txt · Last modified: 2023/02/23 07:08 by lan