Differences

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--- howto:roi_fitting_using_the_flim_script [2014/06/18 15:42] – admin
+++ howto:roi_fitting_using_the_flim_script [2014/06/18 15:43] – admin
@@ Line 13: / Line 13: @@
 {{ youtube>g6uyvLQW4nY?large }}
-==== Step-by-Step Tutorial ====
+===== Step-by-Step =====
-=== Select a file and start the script ===
+==== Select a file and start the script ====
   * Start [[products:SymPhoTime64|SymPhoTime 64]] software.
@@ Line 56: / Line 56: @@
 **Response:** The color scale in the image adapts and lifetime contrasts in the pollen become visible.
-=== Select 3 Regions of Interest (ROI) ===
+==== Select 3 Regions of Interest (ROI) ====
@@ Line 128: / Line 128: @@
 **ASCII** exports the image as ASCII array using a text file format. Thereby one array encodes the intensity values, while a second array encodes the Fast Lifetime values. After removal of the header and the unwanted array, individual arrays can be imported in graphic programs, e.g. ImageJ as a text image. These images contain the full count resolution of the raw image.
-=== Perform a Lifetime Fit on the Selected Regions (ROIs) ===
+==== Perform a Lifetime Fit on the Selected Regions (ROIs) ====
   * In the decay form on the lower left, select n-exponential reconvolution as fitting model.
@@ Line 205: / Line 205: @@
 {{ roi_fitting_using_the_flim_script_Image_25.png?600 }}
-**Respond:** A window opens, asking for a file name to store the fitting values. The data are stored as a .dat file.
+**Response:** A window opens, asking for a file name to store the fitting values. The data are stored as a ''.dat'' file.
 {{ roi_fitting_using_the_flim_script_Image_26.png?600 }}
-  * The .dat file contains the fitting values as table and can be opened e.g. using the Notepad program. They can also be imported into Excel or similar programs.
+  * The ''.dat'' file contains the fitting values as table and can be opened e.g. using the Notepad program. They can also be imported into Excel or similar programs.
 {{ roi_fitting_using_the_flim_script_Image_27.png?600 }}