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--- howto:roi_fitting_using_the_flim_script [2014/06/18 15:40] – admin
+++ howto:roi_fitting_using_the_flim_script [2015/02/25 13:58] – bornemann
@@ Line 3: / Line 3: @@
 ~~NOTOC~~
-====== FLIM ROI fitting ======
+====== FLIM ROI Fitting ======
@@ Line 11: / Line 11: @@
 This tutorial shows step-by-step, how the [[glossary:flim|FLIM]] script of [[products:SymPhoTime64|SymPhoTime 64]] can be used to fit several regions of interest (ROIs), and how to extract and interpret the results. In detail, the FLIM script is started using a daisy pollen image from the "Samples" – workspace, then three regions of interest are defined which are finally fitted.
-==== Step-by-Step Tutorial ====
+{{ youtube>g6uyvLQW4nY?large }}
+===== Step-by-Step =====
-=== Select a file and start the script ===
+==== Select a file and start the script ====
   * Start [[products:SymPhoTime64|SymPhoTime 64]] software.
@@ Line 54: / Line 56: @@
 **Response:** The color scale in the image adapts and lifetime contrasts in the pollen become visible.
-=== Select 3 Regions of Interest (ROI) ===
+==== Select 3 Regions of Interest (ROI) ====
@@ Line 126: / Line 128: @@
 **ASCII** exports the image as ASCII array using a text file format. Thereby one array encodes the intensity values, while a second array encodes the Fast Lifetime values. After removal of the header and the unwanted array, individual arrays can be imported in graphic programs, e.g. ImageJ as a text image. These images contain the full count resolution of the raw image.
-=== Perform a Lifetime Fit on the Selected Regions (ROIs) ===
+==== Perform a Lifetime Fit on the Selected Regions (ROIs) ====
   * In the decay form on the lower left, select n-exponential reconvolution as fitting model.
@@ Line 203: / Line 205: @@
 {{ roi_fitting_using_the_flim_script_Image_25.png?600 }}
-**Respond:** A window opens, asking for a file name to store the fitting values. The data are stored as a .dat file.
+**Response:** A window opens, asking for a file name to store the fitting values. The data are stored as a ''.dat'' file.
 {{ roi_fitting_using_the_flim_script_Image_26.png?600 }}
-  * The .dat file contains the fitting values as table and can be opened e.g. using the Notepad program. They can also be imported into Excel or similar programs.
+  * The ''.dat'' file contains the fitting values as table and can be opened e.g. using the Notepad program. They can also be imported into Excel or similar programs.
 {{ roi_fitting_using_the_flim_script_Image_27.png?600 }}
@@ Line 220: / Line 222: @@
 **Note:**\\
 This tutorial explains only a subset of the functions of the FLIM script. Also check the script explaining pixel fitting ([[howto:Lifetime Fitting using the FLIM Analysis]]).