howto:roi_fitting_using_the_flim_script
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howto:roi_fitting_using_the_flim_script [2014/05/06 14:14] – admin | howto:roi_fitting_using_the_flim_script [2020/05/13 09:42] – [FLIM ROI Fitting] ruckelshausen | ||
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- | {{tag> howto FLIM imaging ROI analysis tutorial}} | + | {{tag> howto FLIM imaging ROI analysis tutorial |
~~NOTOC~~ | ~~NOTOC~~ | ||
- | ====== ROI fitting using the FLIM script | + | ====== ROI Fitting Using the FLIM Analysis |
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This tutorial shows step-by-step, | This tutorial shows step-by-step, | ||
- | ==== Step-by-Step Tutorial ==== | + | {{ youtube> |
+ | ===== Step-by-Step ===== | ||
- | === Select a file and start the script === | + | |
+ | ==== Select a file and start the script | ||
* Start [[products: | * Start [[products: | ||
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**Response: | **Response: | ||
- | === Select 3 Regions of Interest (ROI) === | + | ==== Select 3 Regions of Interest (ROI) ==== |
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**ASCII** exports the image as ASCII array using a text file format. Thereby one array encodes the intensity values, while a second array encodes the Fast Lifetime values. After removal of the header and the unwanted array, individual arrays can be imported in graphic programs, e.g. ImageJ as a text image. These images contain the full count resolution of the raw image. | **ASCII** exports the image as ASCII array using a text file format. Thereby one array encodes the intensity values, while a second array encodes the Fast Lifetime values. After removal of the header and the unwanted array, individual arrays can be imported in graphic programs, e.g. ImageJ as a text image. These images contain the full count resolution of the raw image. | ||
- | === Perform a Lifetime Fit on the Selected Regions (ROIs) === | + | ==== Perform a Lifetime Fit on the Selected Regions (ROIs) |
* In the decay form on the lower left, select n-exponential reconvolution as fitting model. | * In the decay form on the lower left, select n-exponential reconvolution as fitting model. | ||
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{{ roi_fitting_using_the_flim_script_Image_25.png? | {{ roi_fitting_using_the_flim_script_Image_25.png? | ||
- | **Respond:** A window opens, asking for a file name to store the fitting values. The data are stored as a .dat file. | + | **Response:** A window opens, asking for a file name to store the fitting values. The data are stored as a '' |
{{ roi_fitting_using_the_flim_script_Image_26.png? | {{ roi_fitting_using_the_flim_script_Image_26.png? | ||
- | * The .dat file contains the fitting values as table and can be opened e.g. using the Notepad program. They can also be imported into Excel or similar programs. | + | * The '' |
{{ roi_fitting_using_the_flim_script_Image_27.png? | {{ roi_fitting_using_the_flim_script_Image_27.png? | ||
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**Note:**\\ | **Note:**\\ | ||
This tutorial explains only a subset of the functions of the FLIM script. Also check the script explaining pixel fitting ([[howto: | This tutorial explains only a subset of the functions of the FLIM script. Also check the script explaining pixel fitting ([[howto: | ||
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howto/roi_fitting_using_the_flim_script.txt · Last modified: 2023/02/23 07:08 by lan