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howto:lifetime_fitting_using_the_tcpsc_fitting_script [2014/06/18 16:11] adminhowto:lifetime_fitting_using_the_tcpsc_fitting_script [2023/09/07 22:43] (current) – [Step-by-Step Tutorial] peter
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-This tutorial shows step-by-step, how to fit the lifetime of a measured sample. As an example, a single exponential reconvolution fit is used to determine the lifetime of ATTO655 diluted in water.+This tutorial shows step-by-step, how to fit a selected model to a decay curve in order to get the lifetime of a measured sample. As an example, a single exponential reconvolution fit is used to determine the lifetime of ATTO655 diluted in water.
  
 {{ youtube>WupJkKpUVV8?large }} {{ youtube>WupJkKpUVV8?large }}
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 {{ lifetime_fitting_using_the_tcpsc_fitting_script_Image_6.png?600 }} {{ lifetime_fitting_using_the_tcpsc_fitting_script_Image_6.png?600 }}
  
-**Note:** The software offers the possibility to fit the data using a n-exponential tailfit or a n-exponential reconvolution fit. A tailfit can be used when the fitted lifetimes are significantly longer than the instrument response function. Still a reconvolution fit is usually preferable, because the complete decay is fitted, while for a tailfit, the start of the fitting range is usually a bit arbitrary.\\ +**Note:** The software offers the possibility to use a n-exponential tailfit or a n-exponential reconvolution model fit. A tailfit can be used when the expected lifetimes are significantly longer than the FWHM of instrument response function. Still a reconvolution fit is usually preferable, because the complete decay, including its rising edge is analyzed, while for a tailfit, the start of the fitting range is usually a bit arbitrary.\\ 
-For explanation about the fitting model and the used equations, click on the "Help" button next to the selected model. This opens a help window containing the fitting equation and the explanation of the different parameters.+For explanation about the fitting model and the used equations, click on the "Help" button next to the selected model. This opens a help window containing the fitted model l equation and the explanation of the different parameters.
  
   * Click: "Initial Fit" (marked in orange).   * Click: "Initial Fit" (marked in orange).
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 The χ²-value approaches 1.\\ The χ²-value approaches 1.\\
 The calculated fitting values are reasonable.\\ The calculated fitting values are reasonable.\\
-Usually the fitting model with least parameters is selected.\\+ 
 +Usually the fitting model using the smallest amount of adjustable parameters is selected.\\
 => In this example, the fit is already sufficient. => In this example, the fit is already sufficient.
  
howto/lifetime_fitting_using_the_tcpsc_fitting_script.1403107861.txt.gz · Last modified: 2014/06/18 16:11 by admin