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howto:lifetime-fitting_using_the_flim_script [2021/03/23 10:41] – [Introduction ​] rezvanihowto:lifetime-fitting_using_the_flim_script [2021/10/19 12:36] – [Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5)] rezvani
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 {{tag>howto tutorial FLIM analysis SPT MT LSM_upgrade}} {{tag>howto tutorial FLIM analysis SPT MT LSM_upgrade}}
  
-====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5) ======+====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5 and above) ======
  
 ===== Introduction ​===== ===== Introduction ​=====
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   * Set the same limit for A2 and A3.\\   * Set the same limit for A2 and A3.\\
   * Uncheck the boxes for the individual lifetime components, Bkgr(Decay), Bkgr(IRF) and Shift(IRF) in order to fix these values, IRF and Background. Then in the pixel-by-pixel fit, these values are kept constant. Do NOT click "Initial Fit" or "Fit" at this stage!\\   * Uncheck the boxes for the individual lifetime components, Bkgr(Decay), Bkgr(IRF) and Shift(IRF) in order to fix these values, IRF and Background. Then in the pixel-by-pixel fit, these values are kept constant. Do NOT click "Initial Fit" or "Fit" at this stage!\\
-{{ howto:parameterfix_roi0_kidney_2.png?400 |}}+ 
 +{{ :writingroom:3comp-exp-kidney.png?400 |}}
  
   * Click "FLIM Fit" in the upper panel.\\   * Click "FLIM Fit" in the upper panel.\\
howto/lifetime-fitting_using_the_flim_script.txt · Last modified: 2023/08/03 14:16 by lan