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--- howto:lifetime-fitting_using_the_flim_script [2020/05/13 09:38] – [Lifetime-Fitting using the FLIM Analysis (updated for SymPhoTime v2.5)] ruckelshausen
+++ howto:lifetime-fitting_using_the_flim_script [2023/02/17 12:42] – admin
@@ Line 1: / Line 1: @@
-{{tag>howto tutorial FLIM analysis SPT MT LSM_upgrade}}
+{{tag>howto tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}}
-====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5) ======
+====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above) ======
 ===== Introduction =====
-This tutorial shows step-by-step, how the FLIM script of SymPhoTime 64 (V2.5) can be used to perform a pixel-by-pixel lifetime fit of a FLIM image and how to extract and read the results. In detail, the FLIM script is started using an image of mouse kidney tissue in the "Tutorials" workspace, a suited fitting model is chosen and a pixel-by-pixel fit is performed.
+This tutorial shows step-by-step, how the FLIM script of SymPhoTime 64 (V2.5) can be used to perform a pixel-by-pixel lifetime fit of a FLIM image and how to extract and read the results. In detail, the FLIM script is started using an image of mouse kidney tissue in the "Tutorials" workspace,[[https://figshare.com/s/4957fcfa684daef86c23]], a suited fitting model is chosen and a pixel-by-pixel fit is performed.
@@ Line 151: / Line 151: @@
   * Set the same limit for A2 and A3.\\
   * Uncheck the boxes for the individual lifetime components, Bkgr(Decay), Bkgr(IRF) and Shift(IRF) in order to fix these values, IRF and Background. Then in the pixel-by-pixel fit, these values are kept constant. Do NOT click "Initial Fit" or "Fit" at this stage!\\
-{{ howto:parameterfix_roi0_kidney_2.png?400 |}}
+{{ :writingroom:3comp-exp-kidney.png?400 |}}
   * Click "FLIM Fit" in the upper panel.\\