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howto:how_to_work_with_the_instrument_response_function_irf

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howto:how_to_work_with_the_instrument_response_function_irf [2020/05/12 15:25]
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howto:how_to_work_with_the_instrument_response_function_irf [2020/05/13 11:05] (current)
buschmann
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 For IRF Measurements,​ several strategies are possible which are described here: https://​www.tcspc.com/​doku.php/​howto:​how_to_measure_the_instrument_response_function_irf  ​ For IRF Measurements,​ several strategies are possible which are described here: https://​www.tcspc.com/​doku.php/​howto:​how_to_measure_the_instrument_response_function_irf  ​
    
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 For the further analysis it is assumed that: For the further analysis it is assumed that:
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-Note:+**Note:**
  
   * The  green area is used for the timegate.   * The  green area is used for the timegate.
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-Note:+**Note:**
  
   * In general, an indicator of the achievable time resolution of the system is the FWHM (full width of half maximum) of an IRF-measurement. For PicoQuant systems it´s usually in the range of 100-200 ps, even up to 300 ps in some cases (Please see `System Specification File` which is left by installation in your lab. The main influence is due to the settings of the PDL or SEPIA -Unit, as operating the laser with full power will increase the pulse width and IRF. Also the type of detector is important. Finally the type of TCSPC-unit used has an influence. Apart from the laser (unless you use a TiSa), however, all the other factors can be considered constant. For SPAD detectors, the IRF also depends on the detection wavelength).   * In general, an indicator of the achievable time resolution of the system is the FWHM (full width of half maximum) of an IRF-measurement. For PicoQuant systems it´s usually in the range of 100-200 ps, even up to 300 ps in some cases (Please see `System Specification File` which is left by installation in your lab. The main influence is due to the settings of the PDL or SEPIA -Unit, as operating the laser with full power will increase the pulse width and IRF. Also the type of detector is important. Finally the type of TCSPC-unit used has an influence. Apart from the laser (unless you use a TiSa), however, all the other factors can be considered constant. For SPAD detectors, the IRF also depends on the detection wavelength).
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-In this example channel 2 was selected, now the data can be "saved as IRF" ,and be imported for future analysis accordingly.+In this example channel 2 was selected, now the data can be "saved as IRF", and be imported for future analysis accordingly. Please check the different FLIM-related tutorials for more information about the fitting.
  
  
howto/how_to_work_with_the_instrument_response_function_irf.txt · Last modified: 2020/05/13 11:05 by buschmann