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--- howto:how_to_work_with_the_instrument_response_function_irf [2020/05/13 09:05] – buschmann
+++ howto:how_to_work_with_the_instrument_response_function_irf [2023/02/17 12:41] (current) – admin
@@ Line 1: / Line 1: @@
-{{tag>howto IRF tutorial analysis MT LSM_upgrade}}
+{{tag>howto IRF tutorial analysis MicroTime LSM_upgrade}}
@@ Line 35: / Line 35: @@
-. First the measured IRF needs to be made accessible for being used in the analysis. Therefore, highlight the file with the measured IRF (single mouse click on the .ptu-file)\\
+. First the measured IRF needs to be made accessible for being used in the analysis. Therefore, highlight the file with the measured IRF (single mouse click on the .ptu-file)\\ An example measured IRF can be found in Tutorials workspace [[https://figshare.com/s/4957fcfa684daef86c23]].\\
 . In Analysis tab, under TCSPC, find `TCSPC Decay`. Click Start;
@@ Line 67: / Line 67: @@
 **Note:**
-  * In general, an indicator of the achievable time resolution of the system is the FWHM (full width of half maximum) of an IRF-measurement. For PicoQuant systems it´s usually in the range of 100-200 ps, even up to 300 ps in some cases (Please see `System Specification File` which is left by installation in your lab. The main influence is due to the settings of the PDL or SEPIA -Unit, as operating the laser with full power will increase the pulse width and IRF. Also the type of detector is important. Finally the type of TCSPC-unit used has an influence. Apart from the laser (unless you use a TiSa), however, all the other factors can be considered constant. For SPAD detectors, the IRF also depends on the detection wavelength).
+  * In general, an indicator of the achievable time resolution of the system is the FWHM (full width of half maximum) of an IRF-measurement. For PicoQuant systems it´s usually in the range of 100-400 ps, depending on detector, electronics and laser settings (Please see `System Specification File` which is left by installation in your lab. The main influence is due to the settings of the PDL or SEPIA-Unit, as operating the laser with full power will increase the pulse width and IRF. Also the type of detector is important. Finally the type of TCSPC-unit used has an influence. Apart from the laser (unless you use a TiSa), however, all the other factors can be considered constant. For SPAD detectors, the IRF also depends on the detection wavelength).
-Select the linear range for measured curve (on top right), right click, activate `Data Reader`, and try to find FWHM and see how broad it is (in this case ~185 ps). ((This IRF has been measured with quenched fluorescein solution, 485 nm pulsed excitation LDH series laser (running at 40 MHz repetition rate), MultiHarp 150 TCSPC card with 80 ps resolution and a PMA Hybrid detector using a long pass filter (> 500 nm).))
+Select the linear range for measured curve (on top right), right click, activate `Data Reader`, and try to find FWHM and see how broad it is. ((This IRF has been measured with quenched fluorescein solution, 485 nm pulsed excitation LDH series laser (running at 40 MHz repetition rate), MultiHarp 150 TCSPC card with 80 ps resolution and a PMA Hybrid detector using a long pass filter (> 500 nm).))