howto:how_to_work_with_the_instrument_response_function_irf
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| howto:how_to_work_with_the_instrument_response_function_irf [2020/05/12 13:25] – ↷ Links adapted because of a move operation ruckelshausen | howto:how_to_work_with_the_instrument_response_function_irf [2020/05/13 09:05] – buschmann | ||
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| For IRF Measurements, | For IRF Measurements, | ||
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| For the further analysis it is assumed that: | For the further analysis it is assumed that: | ||
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| - | Note: | + | **Note:** |
| * The green area is used for the timegate. | * The green area is used for the timegate. | ||
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| - | Note: | + | **Note:** |
| * In general, an indicator of the achievable time resolution of the system is the FWHM (full width of half maximum) of an IRF-measurement. For PicoQuant systems it´s usually in the range of 100-200 ps, even up to 300 ps in some cases (Please see `System Specification File` which is left by installation in your lab. The main influence is due to the settings of the PDL or SEPIA -Unit, as operating the laser with full power will increase the pulse width and IRF. Also the type of detector is important. Finally the type of TCSPC-unit used has an influence. Apart from the laser (unless you use a TiSa), however, all the other factors can be considered constant. For SPAD detectors, the IRF also depends on the detection wavelength). | * In general, an indicator of the achievable time resolution of the system is the FWHM (full width of half maximum) of an IRF-measurement. For PicoQuant systems it´s usually in the range of 100-200 ps, even up to 300 ps in some cases (Please see `System Specification File` which is left by installation in your lab. The main influence is due to the settings of the PDL or SEPIA -Unit, as operating the laser with full power will increase the pulse width and IRF. Also the type of detector is important. Finally the type of TCSPC-unit used has an influence. Apart from the laser (unless you use a TiSa), however, all the other factors can be considered constant. For SPAD detectors, the IRF also depends on the detection wavelength). | ||
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| - | In this example channel 2 was selected, now the data can be "saved as IRF" ,and be imported for future analysis accordingly. | + | In this example channel 2 was selected, now the data can be "saved as IRF", and be imported for future analysis accordingly. Please check the different FLIM-related tutorials for more information about the fitting. |
howto/how_to_work_with_the_instrument_response_function_irf.txt · Last modified: 2023/02/17 12:41 by admin