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howto:how_to_work_with_the_instrument_response_function_irf [2020/05/12 13:17] ruckelshausenhowto:how_to_work_with_the_instrument_response_function_irf [2020/05/13 09:05] buschmann
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  and width of the IRF. This width is  an indicator the timing resolution of the instrument.  and width of the IRF. This width is  an indicator the timing resolution of the instrument.
  
-{{ :writingroom:irf.png?500 |}}+{{ howto:irf.png?500 |}}
  
  
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 For IRF Measurements, several strategies are possible which are described here: https://www.tcspc.com/doku.php/howto:how_to_measure_the_instrument_response_function_irf   For IRF Measurements, several strategies are possible which are described here: https://www.tcspc.com/doku.php/howto:how_to_measure_the_instrument_response_function_irf  
    
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- 
  
 For the further analysis it is assumed that: For the further analysis it is assumed that:
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 2. In Analysis tab, under TCSPC, find `TCSPC Decay`. Click Start; 2. In Analysis tab, under TCSPC, find `TCSPC Decay`. Click Start;
  
-{{ :writingroom:tcspc_decay_gui.png?400 |}}+{{ howto:tcspc_decay_gui.png?400 |}}
 Response: you see this kind of curve: Response: you see this kind of curve:
-{{ :writingroom:irf_decay_before_timegate.png?800 |}}+{{ howto:irf_decay_before_timegate.png?800 |}}
 (Before selecting a detection channel the peak is above 100000, so fine for this step)\\ (Before selecting a detection channel the peak is above 100000, so fine for this step)\\
 3. Select the proper detection channel (1 or 2) and Click”Calculate Decay”. The decay is then updated.\\ 3. Select the proper detection channel (1 or 2) and Click”Calculate Decay”. The decay is then updated.\\
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-Note:+**Note:**
  
   * The  green area is used for the timegate.   * The  green area is used for the timegate.
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 As a result you will see:  As a result you will see: 
  
-{{ :writingroom:irf_select_channel_timegate.png?800 |}}+{{ howto:irf_select_channel_timegate.png?800 |}}
 6. Click on “Export Decay as IRF”. Rename the file accordingly. The IRF can now be used for the fitting. 6. Click on “Export Decay as IRF”. Rename the file accordingly. The IRF can now be used for the fitting.
  
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-Note:+**Note:**
  
   * In general, an indicator of the achievable time resolution of the system is the FWHM (full width of half maximum) of an IRF-measurement. For PicoQuant systems it´s usually in the range of 100-200 ps, even up to 300 ps in some cases (Please see `System Specification File` which is left by installation in your lab. The main influence is due to the settings of the PDL or SEPIA -Unit, as operating the laser with full power will increase the pulse width and IRF. Also the type of detector is important. Finally the type of TCSPC-unit used has an influence. Apart from the laser (unless you use a TiSa), however, all the other factors can be considered constant. For SPAD detectors, the IRF also depends on the detection wavelength).   * In general, an indicator of the achievable time resolution of the system is the FWHM (full width of half maximum) of an IRF-measurement. For PicoQuant systems it´s usually in the range of 100-200 ps, even up to 300 ps in some cases (Please see `System Specification File` which is left by installation in your lab. The main influence is due to the settings of the PDL or SEPIA -Unit, as operating the laser with full power will increase the pulse width and IRF. Also the type of detector is important. Finally the type of TCSPC-unit used has an influence. Apart from the laser (unless you use a TiSa), however, all the other factors can be considered constant. For SPAD detectors, the IRF also depends on the detection wavelength).
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-{{ :writingroom:irf_linear_scale_fwhm.png?800 |}}+{{ howto:irf_linear_scale_fwhm.png?800 |}}
  
  
-In this example channel 2 was selected, now the data can be "saved as IRF" ,and be imported for future analysis accordingly.+In this example channel 2 was selected, now the data can be "saved as IRF", and be imported for future analysis accordingly. Please check the different FLIM-related tutorials for more information about the fitting.
  
  
howto/how_to_work_with_the_instrument_response_function_irf.txt · Last modified: 2023/02/17 12:41 by admin