howto:how_to_work_with_the_instrument_response_function_irf
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howto:how_to_work_with_the_instrument_response_function_irf [2020/05/12 13:17] – ruckelshausen | howto:how_to_work_with_the_instrument_response_function_irf [2020/05/13 09:05] – buschmann | ||
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and width of the IRF. This width is an indicator the timing resolution of the instrument. | and width of the IRF. This width is an indicator the timing resolution of the instrument. | ||
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For IRF Measurements, | For IRF Measurements, | ||
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For the further analysis it is assumed that: | For the further analysis it is assumed that: | ||
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2. In Analysis tab, under TCSPC, find `TCSPC Decay`. Click Start; | 2. In Analysis tab, under TCSPC, find `TCSPC Decay`. Click Start; | ||
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Response: you see this kind of curve: | Response: you see this kind of curve: | ||
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(Before selecting a detection channel the peak is above 100000, so fine for this step)\\ | (Before selecting a detection channel the peak is above 100000, so fine for this step)\\ | ||
3. Select the proper detection channel (1 or 2) and Click”Calculate Decay”. The decay is then updated.\\ | 3. Select the proper detection channel (1 or 2) and Click”Calculate Decay”. The decay is then updated.\\ | ||
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* The green area is used for the timegate. | * The green area is used for the timegate. | ||
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As a result you will see: | As a result you will see: | ||
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6. Click on “Export Decay as IRF”. Rename the file accordingly. The IRF can now be used for the fitting. | 6. Click on “Export Decay as IRF”. Rename the file accordingly. The IRF can now be used for the fitting. | ||
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* In general, an indicator of the achievable time resolution of the system is the FWHM (full width of half maximum) of an IRF-measurement. For PicoQuant systems it´s usually in the range of 100-200 ps, even up to 300 ps in some cases (Please see `System Specification File` which is left by installation in your lab. The main influence is due to the settings of the PDL or SEPIA -Unit, as operating the laser with full power will increase the pulse width and IRF. Also the type of detector is important. Finally the type of TCSPC-unit used has an influence. Apart from the laser (unless you use a TiSa), however, all the other factors can be considered constant. For SPAD detectors, the IRF also depends on the detection wavelength). | * In general, an indicator of the achievable time resolution of the system is the FWHM (full width of half maximum) of an IRF-measurement. For PicoQuant systems it´s usually in the range of 100-200 ps, even up to 300 ps in some cases (Please see `System Specification File` which is left by installation in your lab. The main influence is due to the settings of the PDL or SEPIA -Unit, as operating the laser with full power will increase the pulse width and IRF. Also the type of detector is important. Finally the type of TCSPC-unit used has an influence. Apart from the laser (unless you use a TiSa), however, all the other factors can be considered constant. For SPAD detectors, the IRF also depends on the detection wavelength). | ||
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- | In this example channel 2 was selected, now the data can be "saved as IRF" ,and be imported for future analysis accordingly. | + | In this example channel 2 was selected, now the data can be "saved as IRF", and be imported for future analysis accordingly. Please check the different FLIM-related tutorials for more information about the fitting. |
howto/how_to_work_with_the_instrument_response_function_irf.txt · Last modified: 2023/02/17 12:41 by admin