howto:how_to_measure_the_instrument_response_function_irf
Differences
This shows you the differences between two versions of the page.
Both sides previous revisionPrevious revisionNext revision | Previous revision | ||
howto:how_to_measure_the_instrument_response_function_irf [2023/09/07 22:48] – [Make sure that the detection count rate is much lower than the count rate used for fluorescence decay measurement.] peter | howto:how_to_measure_the_instrument_response_function_irf [2023/09/07 22:55] (current) – [Using samples with ultrafast decay] peter | ||
---|---|---|---|
Line 10: | Line 10: | ||
{{youtube> | {{youtube> | ||
+ | \\ | ||
+ | ==== Low count rate during IRF measurements is important! ==== | ||
- | === Low count rate during | + | Make sure that the detection count rate is much lower than the count rate during |
- | **Note: | + | If the IRF should be measured |
- | + | ||
- | If the IRF should be measured on a microscope system with SPAD detectors, | + | |
===== Using samples with ultrafast decay ===== | ===== Using samples with ultrafast decay ===== | ||
- | Some detectors (particularly SPADs) have wavelength dependent timing response. In this case an IRF recorded at the excitation wavelength may not be useful for precise reconvolution. The solution is to acquire the IRF at the fluorescence wavelength, or at least spectrally closer to the fluorescence emission. | + | Some detectors (particularly |
==== General recipe ==== | ==== General recipe ==== |
howto/how_to_measure_the_instrument_response_function_irf.txt · Last modified: 2023/09/07 22:55 by peter