howto:how_to_measure_the_instrument_response_function_irf
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howto:how_to_measure_the_instrument_response_function_irf [2023/09/07 22:47] – [How to Measure the Instrument Response Function (IRF)] peter | howto:how_to_measure_the_instrument_response_function_irf [2023/09/07 22:55] (current) – [Using samples with ultrafast decay] peter | ||
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+ | ==== Low count rate during IRF measurements is important! ==== | ||
- | ==== Make sure that the detection count rate is much lower than the count rate used for fluorescence decay measurement. | + | Make sure that the detection count rate is much lower than the count rate during a fluorescence decay measurement. |
- | **Note:** Diluting the scattering solution is better than using grey filters. Ideal is when the decay and the IRF are recorded at the same [[glossary: | + | If the IRF should be measured |
- | + | ||
- | If the IRF should be measured on a microscope system with SPAD detectors, | + | |
===== Using samples with ultrafast decay ===== | ===== Using samples with ultrafast decay ===== | ||
- | Some detectors (particularly SPADs) have wavelength dependent timing response. In this case an IRF recorded at the excitation wavelength may not be useful for precise reconvolution. The solution is to acquire the IRF at the fluorescence wavelength, or at least spectrally closer to the fluorescence emission. | + | Some detectors (particularly |
==== General recipe ==== | ==== General recipe ==== |
howto/how_to_measure_the_instrument_response_function_irf.txt · Last modified: 2023/09/07 22:55 by peter