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--- howto:how_to_measure_the_instrument_response_function_irf [2022/01/24 10:22] – [IRF measurement with KI and Erythrosine B] rezvani
+++ howto:how_to_measure_the_instrument_response_function_irf [2023/02/23 07:05] – lan
@@ Line 11: / Line 11: @@
 ==== Make sure that the detection count rate is much lower than the count rate used for fluorescence decay measurement. ====
-Diluting the scattering solution is better than using grey filters. Ideal is when the decay and the IRF are recorded at the same [[glossary:differential count rate]] (and NOT at the same average count rates).
+**Note:** Diluting the scattering solution is better than using grey filters. Ideal is when the decay and the IRF are recorded at the same [[glossary:differential count rate]] (and NOT at the same average count rates).
 If the IRF should be measured on a microscope system with SPAD detectors, in the UV range also the Raman-scattering of water can be used. E.g. the Raman scattering can be recorded with a HQ480/40 bandpass filter, if a 405nm diode is used. This method is less suited for long wavelengths, as the Raman efficiency decreases. The water must be very pure in order not to capture any fluorescence.